Western Blot (WB)
(Western blot analysis of HSF4 on human HSF4 recombinant protein using anti-HSF4 antibody at 1/1, 000 dilution.)
Mouse anti-Human HSF4 Monoclonal Antibody | anti-HSF4 antibody
HSF4 Antibody
FC/FACS: 1:100-1:200
Western Blot (WB)
(Western blot analysis of HSF4 on human HSF4 recombinant protein using anti-HSF4 antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of HSF4 on human HSF4 recombinant protein using anti-HSF4 antibody at 1/1, 000 dilution.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HeLa cells with HSF4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HeLa cells with HSF4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
NCBI and Uniprot Product Information
NCBI Description
Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
HSF4: DNA-binding protein that specifically binds heat shock promoter elements (HSE). Isoform HSF4A represses transcription while the isoform HSF4B activates transcription. Defects in HSF4 are the cause of cataract, zonular (CZ). A form of zonular cataract. Zonular or lamellar cataracts are opacities, broad or narrow, usually consisting of powdery white dots affecting only certain layers or zones between the cortex and nucleus of an otherwise clear lens. The opacity may be so dense as to render the entire central region of the lens completely opaque, or so translucent that vision is hardly if at all impeded. Zonular cataracts generally do not involve the embryonic nucleus, though sometimes they involve the fetal nucleus. Usually sharply separated from a clear cortex outside them, they may have projections from their outer edges known as riders or spokes. Defects in HSF4 are the cause of cataract Marner type (CAM). A form of cataract with variable and progressive opacities. Affected individuals present with zonular cataract, although some have nuclear, anterior polar, or stellate cataract. Finger malformation is observed in some kindreds. Belongs to the HSF family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor; DNA-binding; Cell development/differentiation
Chromosomal Location of Human Ortholog: 16q21
Cellular Component: nucleoplasm
Molecular Function: sequence-specific DNA binding; transcription factor activity; transcription corepressor activity; protein phosphatase binding
Biological Process: histone H3-K9 demethylation; camera-type eye development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; cell development
Disease: Cataract 5, Multiple Types
Research Articles on HSF4
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Product Notes
The HSF4 hsf4 (Catalog #AAA9435328) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HSF4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSF4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the HSF4 hsf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSF4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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