Mouse anti-Human HRG/HPRG Monoclonal Antibody | anti-HRG antibody

Anti-HRG/HPRG Antibody, Mouse Monoclonal

Cat. Num. AAA8104125

• Gene Names: HRG; HPRG; HRGP; THPH11
• Reactivity: Human
• Applications: ELISA, ELISA
• Purity: Protein A
• Synonyms: HRG/HPRG; Monoclonal Antibody; Anti-HRG/HPRG Antibody; Mouse Monoclonal; HPRG/HGR Antibody; Mouse MAb; histidine-rich glycoprotein; Anti-HPRG Antibody; Anti-HRGP Antibody; Anti-THPH11 Antibody; anti-HRG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 2D9D4
• Specificity: Human HPRG/HRG
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-HRG antibody: ELISA (EIA), ELISA (EIA) (Cap)
• Application Notes: ELISA: 1:1000-1:2000; ELISA (Cap): 1:250-1:2000; ELISA (Cap): This antibody will detect Human HPRG/HRG in ELISA pair set.; In a sandwich ELISA, it can be used as capture antibody.
• Immunogen: Recombinant Human HPRG protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HPRG (rhHPRG). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-HRG antibody

Histidine-rich glycoprotein, also known as HRG and HPRG, is a glycoprotein located in plasma and platelets and contains an unusually large amount of histidine and proline. In humans, five distinct domains are recognized in the mature HPRG molecule. There are two N-terminal cystatin-like modules (aa 19-254) and one His-Pro-rich region (aa 350-497) that is flanked by two Pro-rich segments (aa 276-321 and 498-525). The His-Pro-rich region contains 10 tandem repeats with an HHPHG motif, and the N-and C-termini are linked by a disulfide bond. The specific functions of HRG remain unclear, but it is known that the protein binds heme, dyes, and divalent metal ions. It inhibits rosette formation and interacts with heparin, thrombospondin, and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis, and the reduction of inhibition of coagulation indicate a potential prothrombotic effect. HPRG is evolutionarily, functionally, and structurally related to cleaved high molecular weight kininogen (HKa), an anti-angiogenic polypeptide that stimulates apoptosis of proliferating endothelial cells through binding to cell-surface tropomyosin. The antiangiogenic activity of the multidomain plasma protein HPRG is localized to its histidine-proline-rich (H/P) domain and has recently been shown to be mediated, at least partially, through binding to cell-surface tropomyosin in fibroblast growth factor-2-activated endothelial cells.

References

Guan X, et al. (2004) Histidine-proline rich glycoprotein (HPRG) binds and transduces anti-angiogenic signals through cell surface tropomyosin on endothelial cells. Thromb Haemost. 92(2): 403-12.Doate F, et al. (2004) Peptides derived from the histidine-proline domain of the histidine-proline-rich glycoprotein bind to tropomyosin and have antiangiogenic and antitumor activities. Cancer Res. 64(16): 5812-7.

NCBI and Uniprot Product Information

• NCBI GI #: 4504489
• NCBI GeneID: 3273
• NCBI Accession #: NP_000403.1
• NCBI GenBank Nucleotide #: NM_000412.4
• UniProt Accession #: P04196; B9EK35; D3DNU7
• Molecular Weight: 59,578 Da
• NCBI Official Full Name: histidine-rich glycoprotein
• NCBI Official Synonym Full Names: histidine rich glycoprotein
• NCBI Official Symbol: HRG
• NCBI Official Synonym Symbols: HPRG; HRGP; THPH11
• NCBI Protein Information: histidine-rich glycoprotein
• UniProt Protein Name: Histidine-rich glycoprotein
• UniProt Gene Name: HRG
• UniProt Synonym Gene Names: HPRG

NCBI Description

This histidine-rich glycoprotein contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. The encoded protein also has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. [provided by RefSeq, Nov 2014]

Uniprot Description

Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha-actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor-associated macrophages, leading to decreased tumor growth and metastasis.