Immunohistochemistry(IHC)
(Human HPV infected cervical tissue statined with Mouse anti HPV 16 monoclonal antibody (MBS460234) at 1:100 for 30 min. at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Mouse HPV 16 Monoclonal Antibody | anti-HPV 16 antibody
Mouse anti HPV 16 Monoclonal Antibody
Reactivity
Virus, Type 16
Applications
Immunohistochemistry, Immunofluorescence
Purity
This mouse IgG is purified by Isotyping-specific Affinity Purification.
Synonyms
HPV 16; Monoclonal Antibody; Mouse anti HPV 16 Monoclonal Antibody; Papillomavirus Type 16 (HPV 16); E5 protein; anti-HPV 16 antibody
Host
Mouse
Reactivity
Virus, Type 16
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
SPM257
Specificity
This antibody recognizes HPV 16 protein. It can be used to detect the HPV infected tissue.
Purity/Purification
This mouse IgG is purified by Isotyping-specific Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH 7.2)containing antibody stabilizer.
Applicable Applications for anti-HPV 16 antibody
IHC(P), IF
Application Notes
Western Blot 0.1-1 ug/mL
ELISA 0.01-0.1 ug/mL
Immunoprecipitation 2-5 ug/mL
IHC 2-5 ug/mL
Flow Cytometry Not tested
ELISA 0.01-0.1 ug/mL
Immunoprecipitation 2-5 ug/mL
IHC 2-5 ug/mL
Flow Cytometry Not tested
Antigen preparation
A recombinant protein of HPV type 16 protein.
Positive control
HPV infected tissue
Cellular location
Nuclear
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Immunohistochemistry(IHC)
(Human HPV infected cervical tissue statined with Mouse anti HPV 16 monoclonal antibody (MBS460234) at 1:100 for 30 min. at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Immunohistochemistry(IHC)
(Human HPV infected cervical tissue statined with Mouse anti HPV 16 monoclonal antibody (MBS460234) at 1:100 for 30 min. at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Related Product Information for anti-HPV 16 antibody
The infection with certain types of HPV, s the so called oncogenic HPV’S including 16,18,31,33 and 35, has been associated as a major risk factor in the subsequent development of cervical cancer. The high risk HPV 16 group is almost equally associated with the cervical intraepithelial neoplasia (CIN) and cervical cancer. HPV 6 and 11 are found mainly in bening cervical lesions while HPV and 18 have been associated with premalignant and malignant cervical lessions. The presence of HPV has been reported in malignant lesions of skin, oral, cavity, tongue and lung. This antibody Papillomavirus type 16 in the nucleus of infected cells or tissues stained by immunohistochemical techniques.
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Product Notes
The HPV 16 (Catalog #AAA460234) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti HPV 16 Monoclonal Antibody reacts with Virus, Type 16 and may cross-react with other species as described in the data sheet. AAA Biotech's HPV 16 can be used in a range of immunoassay formats including, but not limited to, IHC(P), IF. Western Blot 0.1-1 ug/mL ELISA 0.01-0.1 ug/mL Immunoprecipitation 2-5 ug/mL IHC 2-5 ug/mL Flow Cytometry Not tested . Researchers should empirically determine the suitability of the HPV 16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HPV 16, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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