Mouse HP1 alpha Monoclonal Antibody | anti-HP1 alpha antibody

HP1 alpha Monoclonal Antibody

Cat. Num. AAA9611256

• Gene Names: CBX5; HP1; HP1A; HEL25
• Reactivity: Human; Mouse; Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity-chromatography.
• Synonyms: HP1 alpha; Monoclonal Antibody; HP1 alpha Monoclonal Antibody; CBX5 Antibody; Antigen p25; CBX5; CBX5_HUMAN; CG8409; Chromobox 5; Chromobox homolog 5 (HP1 alpha homolog; Drosophila); Chromobox homolog 5; Chromobox protein homolog 5; Epididymis luminal protein 25; HEL25; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1 homolog alpha; HP1 alpha homolog; HP1; HP1A; HP1Hs alpha; Su(var)205;; anti-HP1 alpha antibody

• Host: Mouse
• Reactivity: Human; Mouse; Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: HP1 alpha Monoclonal Antibody detects endogenous levels of total HP1 alpha.
• Purity/Purification: Affinity-chromatography.
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 191

• Applicable Applications for anti-HP1 alpha antibody: Western Blot (WB), Immunohistochemmistry (IHC)
• Application Notes: WB 1:500-1:2000, IHC 1:100-1:1000
• Immunogen: Purified recombinant fragment of human HP1 alpha expressed in E. Coli.
• Fragment: Fab fragment
• Conjugation: Unconjugated
• Storage Buffer: Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C. Stable for 12 months from date of receipt.
• Post Translational Modifications: Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.Ubiquitinated.
• Preparation and Storage: Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 degree C. Stable for 12 months from date of receipt.

Related Product Information for anti-HP1 alpha antibody

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 188035908
• NCBI GeneID: 23468
• NCBI Accession #: NP_001120793.1
• NCBI GenBank Nucleotide #: NP_001120793.1
• UniProt Accession #: P45973
• Molecular Weight: Observed Mol.Wt.: 22kDa. Predicted Mol.Wt.: 23kDa.
• NCBI Official Full Name: chromobox protein homolog 5
• NCBI Official Synonym Full Names: chromobox 5
• NCBI Official Symbol: CBX5
• NCBI Official Synonym Symbols: HP1; HP1A; HEL25
• NCBI Protein Information: chromobox protein homolog 5
• UniProt Protein Name: Chromobox protein homolog 5
• UniProt Gene Name: CBX5
• UniProt Synonym Gene Names: HP1A; HP1 alpha
• UniProt Entry Name: CBX5_HUMAN

NCBI Description

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

HP1 alpha: Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Interacts with SUV420H1 and SUV420H2. Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys- 9'. Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope. Interacts with CHAMP1. Interacts with ASXL1.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: kinetochore; nucleoplasm; PML body; histone methyltransferase complex; transcriptional repressor complex; histone deacetylase complex; nucleolus; nuclear envelope; nuclear heterochromatin; nucleus; chromocenter

Molecular Function: protein binding, bridging; protein binding; protein homodimerization activity; histone deacetylase binding; chromatin binding; methylated histone residue binding

Biological Process: viral reproduction; negative regulation of transcription, DNA-dependent; blood coagulation