Mouse HO-1 Monoclonal Antibody | anti-HO-1 antibody

HO-1 monoclonal antibody (HO-1-2)

Cat. Num. AAA565727

• Gene Names: Hmox1; Ho1; Heox; Hmox; Ho-1; HEOXG; hsp32
• Reactivity: Human, Mouse, Rat, Dog, Pig, Monkey, Guinea pig, Hamster, Rabbit
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Protein G affinity purified.
• Synonyms: HO-1; Monoclonal Antibody; HO-1 monoclonal antibody (HO-1-2); HMOX1; Hsp32; Heat shock protein 32; Heme oxygenase 1; mAb (HO-1-2); anti-HO-1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Dog, Pig, Monkey, Guinea pig, Hamster, Rabbit
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: HO-1-2
• Specificity: Recognizes human, mouse, rat, dog, guinea pig, hamster, monkey, pig and rabbit HO-1. Detects a band of ~32kDa by Western blot.
• Purity/Purification: Protein G affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 289

• Applicable Applications for anti-HO-1 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Flow Cytometry (1:100), Western Blot (1:1'000).; Detects a band of ~32kDa by Western blot.
• Immunogen: Native rat HO-1.
• Preparation and Storage: Long Term: -20 degree C

Western Blot (WB)

(Western blot analysis: Lane 1: MW Marker, Lane 2: HO-1 Recombinant Rat Protein, Lane 3: Rat Liver Microsomes.)

Flow Cytometry (FC/FACS)

(Flow cytometry analysis of human lung cancer A2 cells stained using isotype control antibody (left) and HO-1 (Hsp32) mAb (HO -1-2) (right).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of frozen human prostate section (stressed) stained using HO-1 (Hsp32) mAb (HO-1-2).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of frozen mouse spinal cord with HO-1, mAb (HO-1-2).)

Related Product Information for anti-HO-1 antibody

Heme Oxygenase-1 (HO-1) also known as Hsp32, is the inducible isoform of heme oxygenase that catalyzes the NADPH, oxygen, and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, ferrous iron and biliverdin which is rapidly reduced to bilirubin. These products of the HO reaction have important physiological effects: carbon monoxide is a potent vasodilator and has been implicated to be a physiological regulator of cGMP and vascular tone; biliverdin and its product bilirubin are potent antioxidants; "free" iron increases oxidative stress and regulates the expression of many mRNAs (e.g., DCT-1, ferritin and transferring receptor) by affecting the conformation of iron regulatory protein (IRP)-1 and its binding to iron regulatory elements (IREs) in the 5'- or 3'- UTRs of the mRNAs. To date, three identified heme oxygenase isoforms are part of the HO system that catalyze heme into biliverdin and carbon monoxide. These are inducible HO-1 or Hsp32, constitutive HO-2 that is abundant in the brain and testis, and HO-3 which is related to HO-2 but is the product of a different gene. The HO system is the rate-limiting step in heme degradation and HO activity decreases the levels of heme which is a well known potent catalyst of lipid peroxidation and oxygen radical formation.

Product Categories/Family for anti-HO-1 antibody

Protein Synthesis & Folding; Oxidative Stress

NCBI and Uniprot Product Information

• NCBI GI #: 123448
• NCBI GeneID: 24451
• UniProt Accession #: P06762; Q5BK87
• Molecular Weight: 33,006 Da
• NCBI Official Full Name: Heme oxygenase 1
• NCBI Official Synonym Full Names: heme oxygenase 1
• NCBI Official Symbol: Hmox1
• NCBI Official Synonym Symbols: Ho1; Heox; Hmox; Ho-1; HEOXG; hsp32
• NCBI Protein Information: heme oxygenase 1
• UniProt Protein Name: Heme oxygenase 1
• UniProt Gene Name: Hmox1
• UniProt Synonym Gene Names: HO-1

NCBI Description

catalyzes the oxidative cleavage of heme to biliverdin [RGD, Feb 2006]

Uniprot Description

HMOX1: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. Expressed at higher levels in renal cancer tissue than in normal tissue. Belongs to the heme oxygenase family.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.14.99.3; Oxidoreductase

Chromosomal Location of Human Ortholog: 19p11

Cellular Component: caveola; cytosol; endoplasmic reticulum; nucleolus; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: enzyme binding; heme binding; heme oxygenase (decyclizing) activity; phospholipase D activity; protein binding; protein homodimerization activity; signal transducer activity

Biological Process: angiogenesis; cell death; cellular iron ion homeostasis; cellular response to nutrient; DNA damage response, signal transduction resulting in induction of apoptosis; erythrocyte homeostasis; healing during inflammatory response; heme catabolic process; heme metabolic process; heme oxidation; iron ion homeostasis; negative regulation of cell proliferation; negative regulation of DNA binding; negative regulation of macroautophagy; negative regulation of mast cell cytokine production; negative regulation of mast cell degranulation; negative regulation of neuron apoptosis; negative regulation of smooth muscle cell proliferation; negative regulation of transcription factor activity; phospholipid metabolic process; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of macroautophagy; positive regulation of smooth muscle cell proliferation; protein homooligomerization; regulation of blood pressure; regulation of transcription factor activity; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; response to estrogen stimulus; response to hydrogen peroxide; response to hypoxia; response to nicotine; response to oxidative stress; small GTPase mediated signal transduction