Mouse HMGB1 Monoclonal Antibody | anti-HMGB1 antibody

HMGB1 Monoclonal Antibody

Cat. Num. AAA668025

• Gene Names: HMGB1; HMG1; HMG3; SBP-1
• Reactivity: Human (Predicted Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: HMGB1; Monoclonal Antibody; HMGB1 Monoclonal Antibody; anti-HMGB1 antibody

• Host: Mouse
• Reactivity: Human (Predicted Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, Kappa
• Clone Number: ABM24D3
• Form/Format: Purified (Liquid)
• Concentration: 0.5 mg/ml, 200 µl (varies by lot)
• Sequence Length: 215

• Applicable Applications for anti-HMGB1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Antigen: HMGB1
• Immunogen: A partial recombinant HMGB1 protein (amino acids 1- 200 was used as the immunogen for this antibody
• Preparation and Storage: Store antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles. Expire 1 year from date of purchase.

Western Blot (WB)

(Expression analysis of HMGB1. Anti- HMGB1 antibody (Clone: ABM24D3) was used at 2 µg/ml on HepG2 lysates.)

Flow Cytometry (FC/FACS)

(Intracellular flow analysis of HMGB1 in PBMC (Monocyte) using 0.5 µg/10^6 cells of HMGB1 antibody (Clone: ABM24D3). Green represents isotype control; red represents anti-HMGB1 antibody. Goat anti-mouse PE conjugate was used as secondary.)

Flow Cytometry (FC/FACS) #2

(Intracellular flow analysis of HMGB1 in PBMC (Lymphocyte) using 0.5 µg/10^6 cells of HMGB1 antibody (Clone: ABM24D3). Green represents isotype control; red represents anti-HMGB1 antibody. Goat anti-mouse PE conjugate was used as secondary.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of HMGB1  in adenocarcinoma of stomach using HMGB1 antibody (Clone: ABM24D3) at 1 ug/ml.)

Immunohistochemistry (IHC) #2

(Immunohistochemical analysis of HMGB1  in normal human prostate tissue using HMGB1 antibody (Clone: ABM24D3) at 1 ug/ml.)

Immunohistochemistry (IHC) #3

(Immunohistochemical analysis of HMGB1  in squamous cell carcinoma of esophagus using HMGB1 antibody (Clone: ABM24D3) at 1 ug/ml.)

Immunohistochemistry (IHC) #4

(Immunohistochemical analysis of HMGB1  in normal human spleen tissue using HMGB1 antibody (Clone: ABM24D3) at 1 ug/ml.)

Product Categories/Family for anti-HMGB1 antibody

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4504425
• NCBI GeneID: 3146
• NCBI Accession #: NP_002119.1
• NCBI GenBank Nucleotide #: NM_002128.4
• UniProt Accession #: P09429; Q14321; Q5T7C3; Q6IBE1; A5D8W9
• Molecular Weight: 24,894 Da
• NCBI Official Full Name: high mobility group protein B1
• NCBI Official Synonym Full Names: high mobility group box 1
• NCBI Official Symbol: HMGB1
• NCBI Official Synonym Symbols: HMG1; HMG3; SBP-1
• NCBI Protein Information: high mobility group protein B1; Amphoterin; HMG-1; Sulfoglucuronyl carbohydrate binding protein; high mobility group protein 1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1
• UniProt Protein Name: High mobility group protein B1
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGB1
• UniProt Synonym Gene Names: HMG1; HMG-1
• UniProt Entry Name: HMGB1_HUMAN

Uniprot Description

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: Nuclear receptor co-regulator; DNA repair, damage

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus

Molecular Function: RAGE receptor binding; protein binding; double-stranded DNA binding; cytokine activity; damaged DNA binding; chromatin binding; transcription factor binding; DNA bending activity; transcription factor activity; single-stranded DNA binding; chemoattractant activity

Biological Process: negative regulation of transcriptional preinitiation complex assembly; DNA topological change; V(D)J recombination; apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; base-excision repair, DNA ligation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; innate immune response; DNA ligation during DNA repair; dendritic cell chemotaxis; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; neurite development; positive regulation of DNA binding; cell structure disassembly during apoptosis