Mouse anti-Human HMG20A Monoclonal Antibody | anti-HMG20A antibody

HMG20A (High Mobility Group Protein 20A, HMG Box-containing Protein 20A, HMG Domain-containing Protein 1, HMG Domain-containing Protein HMGX1, HMGX1, HMGXB1) (HRP)

Cat. Num. AAA6152905

• Gene Names: HMG20A; HMGX1; HMGXB1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HMG20A; Monoclonal Antibody; HMG20A (High Mobility Group Protein 20A; HMG Box-containing Protein 20A; HMG Domain-containing Protein 1; HMG Domain-containing Protein HMGX1; HMGX1; HMGXB1) (HRP); anti-HMG20A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 4D5
• Specificity: Recognizes human HMG20A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-HMG20A antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa153-262 from HMG20A (NP_060670) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: LDEADRDKERYMKELEQYQKTEAYKVFSRKTQDRQKGKSHRQDAARQATHDHEKETEVKERSVFDIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRT
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (38.21kD).)

Western Blot (WB)

(Western Blot analysis of HMG20A expression in transfected 293T cell line by HMG20A monoclonal antibody. Lane 1: HMG20A transfected lysate (40.1kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to HMG20A on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])

Testing Data

(Detection limit for recombinant GST tagged HMG20A is 0.03ng/ml as a capture antibody.)

Product Categories/Family for anti-HMG20A antibody

Antibodies; Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 8922633
• NCBI GeneID: 10363
• NCBI Accession #: NP_060670
• NCBI GenBank Nucleotide #: NM_018200
• UniProt Accession #: Q9NP66
• Molecular Weight: 40kDa
• NCBI Official Full Name: high mobility group protein 20A isoform a
• NCBI Official Synonym Full Names: high mobility group 20A
• NCBI Official Symbol: HMG20A
• NCBI Official Synonym Symbols: HMGX1; HMGXB1
• NCBI Protein Information: high mobility group protein 20A
• UniProt Protein Name: High mobility group protein 20A
• Protein Family: High mobility group protein
• UniProt Gene Name: HMG20A
• UniProt Synonym Gene Names: HMGX1; HMGXB1
• UniProt Entry Name: HM20A_HUMAN

Uniprot Description

HMG20A: Plays a role in neuronal differentiation as chromatin- associated protein. Acts as inhibitor of HMG20B. Overcomes the repressive effects of the neuronal silencer REST and induces the activation of neuronal-specific genes. Involved in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 15q24

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; chromatin binding; transcription factor activity

Biological Process: chromatin remodeling; establishment and/or maintenance of chromatin architecture; negative regulation of neuron differentiation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter