Mouse HLA-DRA Monoclonal Antibody | anti-HLA-DRA antibody

HLA-DRA (MHC II) Mouse Monoclonal Antibody

Cat. Num. AAA439450

• Reactivity: Human, Cow, Cat.; Others not known
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: HLA-DRA; Monoclonal Antibody; HLA-DRA (MHC II) Mouse Monoclonal Antibody; DR alpha chain; HLA class II histocompatibility antigen; HLA class II histocompatibility antigen DR alpha chain; HLA DRA; HLA DRA1; HLADRA1; Major histocompatibility complex class II DR alpha; MHC cell surface glycoprotein; MHC class II antigen DRA; anti-HLA-DRA antibody

• Host: Mouse
• Reactivity: Human, Cow, Cat.; Others not known
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: 169-1B5.2
• Specificity: This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional AP's. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 254

• Applicable Applications for anti-HLA-DRA antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml)
• Cellular Localization: Cell Surface
• Immunogen: Human peripheral blood leukocytes
• Hu-Chromosome Location: 6p21.3
• Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Vaughan J. et al. (1982): Proceedings of the second Asia and Oceania histocompatibility workshop conference. Melbourne. p221. Kuramochi T. et al. (1987): Cross-reactivity between human and feline Ia antigens, using a monoclonal antibody HLA-D.m1. Amer. J. Vet. Res. 48:186-188

NCBI and Uniprot Product Information

• NCBI GI #: 52426774
• NCBI GeneID: 3122
• NCBI Accession #: NP_061984.2
• NCBI GenBank Nucleotide #: NM_019111.4
• UniProt Accession #: P01903; Q30160; Q6IAZ1; Q861I2; Q9TP70; A2BET4
• Molecular Weight: 36kDa (alpha chain); 27kDa (beta chain)
• NCBI Official Full Name: HLA class II histocompatibility antigen, DR alpha chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DR alpha chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DRA
• UniProt Synonym Gene Names: HLA-DRA1
• UniProt Entry Name: DRA_HUMAN

Uniprot Description

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.