Mouse anti-Human HLA-DMA Monoclonal Antibody | anti-HLA-DMA antibody

HLA-DMA (HLA Class II Histocompatibility Antigen, DM alpha Chain, MHC Class II Antigen DMA, Really Interesting New Gene 6 Protein, DMA, RING6) APC

Cat. Num. AAA6136988

• Gene Names: HLA-DMA; DMA; HLADM; RING6; D6S222E
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HLA-DMA; Monoclonal Antibody; HLA-DMA (HLA Class II Histocompatibility Antigen; DM alpha Chain; MHC Class II Antigen DMA; Really Interesting New Gene 6 Protein; DMA; RING6) APC; anti-HLA-DMA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3F12-F11
• Specificity: Recognizes human HLA-DMA.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-HLA-DMA antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-261 from human HLA-DMA with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MGHEQNQGAALLQMLPLLWLLPHSWAVPEAPTPMWPDDLQNHTFLHTVYCQDGSPSVGLSEAYDEDQLFFFDFSQNTRVPRLPEFADWAQEQGDAPAILFDKEFCEWMIQQIGPKLDGKIPVSRGFPIAEVFTLKPLEFGKPNTLVCFVSNLFPPMLTVNWQHHSVPVEGFGPTFVSAVDGLSFQAFSYLNFTPEPSDIFSCIVTHEIDRYTAIAYWVPRNALPSDLLENVLCGVAFGLGVLGIIVGIVLIIYFRKPCSGD
• Conjugate: APC
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged HLA-DMA is ~1ng/ml using 127904 as a capture antibody.)

Western Blot (WB)

(Western Blot detection against Immunogen using 127904 (54.45kD).)

Western Blot (WB)

(Western Blot analysis of HLA-DMA expression in U-2 OS using 127904.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of HLA-DMA on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue using 127904 (3ug/ml))

Product Categories/Family for anti-HLA-DMA antibody

Antibodies; HLA

NCBI and Uniprot Product Information

• NCBI GI #: 15030335
• NCBI GeneID: 3108
• Molecular Weight: 29,194 Da
• NCBI Official Full Name: Homo sapiens major histocompatibility complex, class II, DM alpha, mRNA
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DM alpha
• NCBI Official Symbol: HLA-DMA
• NCBI Official Synonym Symbols: DMA; HLADM; RING6; D6S222E
• NCBI Protein Information: HLA class II histocompatibility antigen, DM alpha chain
• Protein Family: HLA class II histocompatibility antigen

NCBI Description

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. [provided by RefSeq, Jul 2008]