Mouse anti-Human HLA Class 2 Antigen DR1 Monoclonal Antibody | anti-HLA-DMB antibody

HLA Class 2 Antigen DR1 (HLA DR1 Class II, HLA-DR1)

Cat. Num. AAA609848

• Gene Names: HLA-DMB; RING7; D6S221E
• Reactivity: Human
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified; Purified
• Synonyms: HLA Class 2 Antigen DR1; Monoclonal Antibody; HLA Class 2 Antigen DR1 (HLA DR1 Class II; HLA-DR1); Anti -HLA Class 2 Antigen DR1 (HLA DR1 Class II; anti-HLA-DMB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: MEM-267
• Specificity: Recognizes human HLA-DR1.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, 15mM sodium azide, pH7.4.

• Applicable Applications for anti-HLA-DMB antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, ELISA and Western Blot.
• Immunogen: Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-HLA-DMB antibody

HLA-DR1 belongs to the HLA class II beta chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC). The beta chain is approximately 26-28kD. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. The antibody MEM-267 specifically binds to the empty but not peptide-loaded form of HLA-DR1.

Product Categories/Family for anti-HLA-DMB antibody

Antibodies; Abs to HLA

NCBI and Uniprot Product Information

• NCBI GI #: 301129275
• NCBI GeneID: 3109
• NCBI Accession #: NP_002109.2
• NCBI GenBank Nucleotide #: NM_002118.4
• UniProt Accession #: P28068; O77936; Q13012; Q29751; Q58ZE2; Q5SNZ8; Q5STC4; Q9XRX2
• Molecular Weight: 28,943 Da
• NCBI Official Full Name: HLA class II histocompatibility antigen, DM beta chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DM beta
• NCBI Official Symbol: HLA-DMB
• NCBI Official Synonym Symbols: RING7; D6S221E
• NCBI Protein Information: HLA class II histocompatibility antigen, DM beta chain; MHC class II HLA-DMB; MHC class II antigen DMB; really interesting new gene 7 protein; MHC class II antigen HLA-DM beta chain; class II histocompatibility antigen, M beta chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DM beta chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DMB
• UniProt Synonym Gene Names: DMB; RING7
• UniProt Entry Name: DMB_HUMAN

NCBI Description

HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DMB: Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Belongs to the MHC class II family.

Protein type: Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: lysosomal membrane; late endosome membrane; integral to membrane; MHC class II protein complex

Biological Process: peptide antigen assembly with MHC class II protein complex; antigen processing and presentation of exogenous peptide antigen via MHC class II; positive regulation of T cell proliferation; immune response; MHC class II protein complex assembly