Mouse HLA Class 1 Antigen B7 Monoclonal Antibody | anti-HLA-A antibody

HLA Class 1 Antigen B7 (PE)

Cat. Num. AAA602446

• Gene Names: HLA-A; HLAA
• Reactivity: Human, Monkey
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: HLA Class 1 Antigen B7; Monoclonal Antibody; HLA Class 1 Antigen B7 (PE); Anti -HLA Class 1 Antigen B7 (PE); anti-HLA-A antibody

• Host: Mouse
• Reactivity: Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3H2215
• Specificity: Recognizes human HLA-B7 antigen. Does not crossreact with HLA-B27 or other related HLA antigens. Clone 3H2215 can be used to distinguish true HLA-B27 positives from HLA-B27 false positives (ie HLA-B7 positive) in the investigation of such diseases as ankylosing spondylitis and anterior uveitis. Species Crossreactivity: Cynomolgus monkey.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide, 5% sucrose. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml sterile ddH2O.

• Applicable Applications for anti-HLA-A antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: Neat. Use 10ul to label 1x10e6 cells or 100ul whole blood.; Optimal working dilutions to be determined by researcher.
• Immunogen: Papain solubilized HLA-B7 antigen.
• Recommended Negative Controls: I1904-79D: IgG1 Murine Negative Control (PE)
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C. Reconstitute by adding sterile ddH2O. Aliquot and store at 4 degree C. DO NOT FREEZE! Reconstituted product is stable for 12 months. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HLA-A antibody

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. The HLA B gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene.

Product Categories/Family for anti-HLA-A antibody

Antibodies; Abs to HLA

NCBI and Uniprot Product Information

• NCBI GI #: 24797067
• NCBI GeneID: 3105
• NCBI Accession #: NP_002107.3
• NCBI GenBank Nucleotide #: NM_002116.7
• UniProt Accession #: P01891; O19673; O19695; O19794; O19795; O43907; O77938; O98010; P10315; P79505; Q9MYA5; Q9MYC4; P01892; P04439; P05534; P10314; P10316; P13746; P16189; P18462; P30443
• Molecular Weight: 40,909 Da
• NCBI Official Full Name: HLA class I histocompatibility antigen, A-1 alpha chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, A
• NCBI Official Symbol: HLA-A
• NCBI Official Synonym Symbols: HLAA
• NCBI Protein Information: HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain
• UniProt Protein Name: HLA class I histocompatibility antigen, A-68 alpha chain
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-A
• UniProt Synonym Gene Names: HLAA
• UniProt Entry Name: 1A68_HUMAN

NCBI Description

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

HLAA: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; early endosome membrane; integral to plasma membrane; endoplasmic reticulum; plasma membrane; MHC class I protein complex; Golgi medial cisterna

Molecular Function: protein binding; peptide antigen binding; beta-2-microglobulin binding; T cell receptor binding; TAP binding; receptor binding

Biological Process: regulation of immune response; detection of bacterium; viral reproduction; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; protection from natural killer cell mediated cytotoxicity; regulation of defense response to virus by virus; positive regulation of interferon-gamma production; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response

Disease: Severe Cutaneous Adverse Reaction, Susceptibility To