Mouse Histone H2B Monoclonal Antibody | anti-Histone H2B antibody

Histone H2B mouse monoclonal antibody

Cat. Num. AAA9611254

• Gene Names: H2BC11; H2BJ; H2B/r; H2BFR; HIST1H2BJ
• Reactivity: Human; Mouse; Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity-chromatography.
• Synonyms: Histone H2B; Monoclonal Antibody; Histone H2B mouse monoclonal antibody; Histone H2B antibody; dJ221C16.8; H2B histone family; member A; H2B/a; H2B/g; H2B/h; H2B/k; H2B/l; H2B1C_HUMAN; H2BFA; HIST1H2BI; Histone 1; H2bg; Histone cluster 1 H2B family member g; Histone cluster 1; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; Histone H2B.g; Histone H2B.h; Histone H2B.k; Histone H2B.l; H2B histone family member R; H2B/r; H2B1J_HUMAN; H2BFR; HIST1H2BJ; Histone 1 H2bj; Histone cluster 1 H2bj; Histone H2B type 1-J; Histone H2B.1; Histone H2B.r; GL105; H2B; H2B histone family member Q; H2B-GL105; H2B.1; H2B/q; H2B2E_HUMAN; H2BFQ; H2BGL105; H2BQ; HIST2H2BE; Histone 2 H2be; histone cluster 2; H2be; histone H2B GL105; histone H2B type 2 E; Histone H2B type 2-E; histone H2B type 2E; Histone H2B-GL105; Histone H2B.q; histone H2BGL105; H2B K; H2B type 12; H2B/b; H2B/c; H2B/d; H2B/e; H2B/f; H2B/j; H2B/n; H2B/s; HIRA-interacting protein 1; HIRA-interacting protein 2; Histone H2B type 1-B; Histone H2B type 1-D; Histone H2B type 1-H; Histone H2B type 1-K; Histone H2B type 1-L; Histone H2B type 1-M; Histone H2B type 1-N; Histone H2B type 1-O; Histone H2B type 2-F; Histone H2B type 3-B; Histone H2B type F-S; Histone H2B.1 B; Histone H2B.2; Histone H2B.b; Histone H2B.c; Histone H2B.d; Histone H2B.e; Histone H2B.f; Histone H2B.j; Histone H2B.n; Histone H2B.s;; anti-Histone H2B antibody

• Host: Mouse
• Reactivity: Human; Mouse; Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Histone H2B mouse monoclonal antibody detects endogenous levels of total Histone H2B.
• Purity/Purification: Affinity-chromatography.
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 126

• Applicable Applications for anti-Histone H2B antibody: Western Blot (WB), Immunohistochemmistry (IHC)
• Application Notes: WB 1:500-1:2000, IHC 1:100-1:1000
• Immunogen: Purified recombinant fragment of human Histone H2B expressed in E. Coli.
• Fragment: Fab fragment
• Conjugation: Unconjugated
• Storage Buffer: Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C. Stable for 12 months from date of receipt.
• Post Translational Modifications: Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity). Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes (By similarity).Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
• Preparation and Storage: Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 degree C. Stable for 12 months from date of receipt.

Related Product Information for anti-Histone H2B antibody

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Similarity: Belongs to the histone H2B family.

NCBI and Uniprot Product Information

• NCBI GI #: 20336754
• NCBI GeneID: 8970
• NCBI Accession #: NP_066402.2
• NCBI GenBank Nucleotide #: NP_066402.2
• UniProt Accession #: P06899
• Molecular Weight: Observed Mol.Wt.: 14kDa. Predicted Mol.Wt.: 14kDa.
• NCBI Official Full Name: histone H2B type 1-J
• NCBI Official Synonym Full Names: H2B clustered histone 11
• NCBI Official Symbol: H2BC11
• NCBI Official Synonym Symbols: H2BJ; H2B/r; H2BFR; HIST1H2BJ
• NCBI Protein Information: histone H2B type 1-J
• UniProt Protein Name: Histone H2B type 1-J
• Protein Family: Histone
• UniProt Gene Name: HIST1H2BJ
• UniProt Synonym Gene Names: H2BFR; H2B/r
• UniProt Entry Name: H2B1J_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015]

Uniprot Description

HIST1H2BJ: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Belongs to the histone H2B family.

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: nucleoplasm; extracellular space; nucleosome; nucleus

Molecular Function: DNA binding; protein heterodimerization activity

Biological Process: nucleosome assembly; defense response to Gram-positive bacterium; establishment and/or maintenance of chromatin architecture; innate immune response in mucosa; antibacterial humoral response