Mouse anti-Human, Mouse Histone H2A.X Monoclonal Antibody | anti-H2AFX antibody

Phospho-Histone H2A.X (Ser139) Antibody

Cat. Num. AAA9215435

• Gene Names: H2AFX; H2AX; H2A.X; H2A/X
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry
• Synonyms: Histone H2A.X; Monoclonal Antibody; Phospho-Histone H2A.X (Ser139) Antibody; Purified Mouse Monoclonal Antibody (Mab); H2A histone family; member X; H2A.X; H2a/x; H2AFX; H2AX; H2AX histone; H2AX_HUMAN; Histone H2AX; anti-H2AFX antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Form/Format: Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
• Sequence Length: 143

• Applicable Applications for anti-H2AFX antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB ~~ 1:2000; ICC ~~ 1:400
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.)

Immunocytochemistry (ICC)

(Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).)

Related Product Information for anti-H2AFX antibody

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.

References

Mannironi C.,et al.Nucleic Acids Res. 17:9113-9126(1989). Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Rogakou E.P.,et al.J. Biol. Chem. 273:5858-5868(1998). Rogakou E.P.,et al.J. Cell Biol. 146:905-916(1999). Paull T.T.,et al.Curr. Biol. 10:886-895(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4504253
• NCBI GeneID: 3014
• NCBI Accession #: NP_002096.1
• NCBI GenBank Nucleotide #: NM_002105.2
• UniProt Accession #: P16104; Q4ZGJ7; Q6IAS5
• Molecular Weight: 15145
• NCBI Official Full Name: histone H2AX
• NCBI Official Synonym Full Names: H2A histone family member X
• NCBI Official Symbol: H2AFX
• NCBI Official Synonym Symbols: H2AX; H2A.X; H2A/X
• NCBI Protein Information: histone H2AX
• UniProt Protein Name: Histone H2AX
• UniProt Gene Name: H2AFX
• UniProt Synonym Gene Names: H2AX; H2a/x
• UniProt Entry Name: H2AX_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]

Uniprot Description

H2AX: a histone that replaces conventional H2A in a subset of nucleosomes. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Phosphorylated on S139 by ATM and DNA-PK in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Dephosphorylation of S139 by PP2A is required for DNA DSB repair. Apparently phosphorylated on Y143 by WSTF, determining the relative recruitment of either DNA repair or pro-apoptotic factors. H2AXpY142 favors the recruitment of pro-apoptotic factors APBB1 and JNK1. In contrast, dephosphorylation of pY143 by EYA phosphatases favors the recruitment of MDC1-containing DNA repair complexes to the tail of phosphorylated pS139. Monoubiquitination of K119 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'K63' linkages by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage.

Protein type: Helicase; DNA-binding; DNA repair, damage

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: male germ cell nucleus; nucleoplasm; XY body; chromosome, telomeric region; condensed nuclear chromosome; nuclear chromatin; replication fork; nucleosome; nucleus

Molecular Function: protein binding; enzyme binding; DNA binding; histone binding; protein heterodimerization activity; damaged DNA binding

Biological Process: establishment and/or maintenance of chromatin architecture; positive regulation of DNA repair; viral reproduction; chromatin silencing; DNA repair; double-strand break repair via homologous recombination; nucleosome assembly; meiotic cell cycle; double-strand break repair; DNA damage checkpoint; spermatogenesis; cerebral cortex development; response to ionizing radiation; response to DNA damage stimulus