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Western Blot (WB)
(Western Blot (WB) analysis of Histone H2A (Hydroxyl-Y39) Rabbit mAb at 1:1000 dilutionLane1:Hela whole cell lysateLane2:NIH/3T3 whole cell lysate)
Rabbit Histone H2A Monoclonal Antibody | anti-H2A antibody
Histone H2A (Hydroxyl-Y39) Rabbit Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Protein A affinity purified
Synonyms
Histone H2A; Monoclonal Antibody; Histone H2A (Hydroxyl-Y39) Rabbit Monoclonal Antibody; Histone H2A type 1-B/E; HIST1H2AB; H2AFM; HIST1H2AE; H2AFA; Histone H2A type 1-C; HIST1H2AC; H2AFL; Histone H2A type 1-J; HIST1H2AJ; H2AFE; anti-H2A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Specificity
This antibody detects endogenous levels of Histone H2A protein only when Hydroxyl at Tyr9.
Purity/Purification
Protein A affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
130
Applicable Applications for anti-H2A antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant antibody.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Histone H2A (Hydroxyl-Y39) Rabbit mAb at 1:1000 dilutionLane1:Hela whole cell lysateLane2:NIH/3T3 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of Histone H2A (Hydroxyl-Y39) Rabbit mAb at 1:1000 dilutionLane1:Hela whole cell lysateLane2:NIH/3T3 whole cell lysate)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2A (Hydroxyl Y39) antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2A (Hydroxyl Y39) antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A (Hydroxyl Y39) antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2A (Hydroxyl Y39) antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A (Hydroxyl Y39) antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A (Hydroxyl Y39) antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2A (Hydroxyl Y39) antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2A (Hydroxyl Y39) antibody. Counter stained with hematoxylin.)
Related Product Information for anti-H2A antibody
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Global mass spectrometric analysis of histone H2A variants/subtypes and their modifications have only recently been carried out. Nine histone H2A subtypes have been identified, including H2A2A and H2A2B. The two main H2A variants, H2AO and H2AC, as well as H2AL, were either acetylated at Lys 5 or phosphorylated at Ser 1. For the replacement histone H2AZ, acetylation at Lys 4 and Lys 7 was found.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~ 15kDa
NCBI Official Full Name
histone H2A type 1-B/E
UniProt Protein Name
Histone H2A type 1-B/E
Protein Family
UniProt Gene Name
HIST1H2AB
UniProt Synonym Gene Names
H2AFM
Uniprot Description
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Product Notes
The H2A hist1h2ab (Catalog #AAA3014086) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2A (Hydroxyl-Y39) Rabbit Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the H2A hist1h2ab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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