Western Blot (WB)
(WB: HeLa cell acid extract probed with H5110-01 (1ug/ml). Arrow indicates Histone H1 (32-33kD).)
Mouse Histone H1 Monoclonal Antibody | anti-HIST1H1E antibody
Histone H1 (PE)
Gene Names
HIST1H1E; H1E; H1.4; H1F4; H1s-4; dJ221C16.5
Reactivity
Bovine, Human
Applications
ELISA, Immunocytochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
Histone H1; Monoclonal Antibody; Histone H1 (PE); anti-HIST1H1E antibody
Host
Mouse
Reactivity
Bovine, Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
0.T.60
Specificity
Recognizes human histone H1 at ~32-33kD. Doublet detected due to hyper-phosphorylation of histone H1 subpopulation. Crossreacts with other histone isoforms in the following order of signal strength: H3, H2b > H4 > H2a, as determined with recombinant protein. Species Crossreactivity: bovine. Predicted to stain nuclei from other animal and plant species.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-HIST1H1E antibody
ELISA (EIA), Immunocytochemistry (ICC), Western Blot (WB)
Immunogen
Nuclei from myeloid leukemia biopsy cells (NM_005322).
Conjugate
PE
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Western Blot (WB)
(WB: HeLa cell acid extract probed with H5110-01 (1ug/ml). Arrow indicates Histone H1 (32-33kD).)
Western Blot (WB)
(WB: HeLa cell acid extract probed with H5110-01 (1ug/ml). Arrow indicates Histone H1 (32-33kD).)
Product Categories/Family for anti-HIST1H1E antibody
References
1. Irie, Y., et al., PNAS USA 100: 5634-5639 (2003). 2. Gui, C.Y. & Dean, A., Mol. Cell. Biol. 21: 1155-1163 (2001). 3. Bhattacharjee, R.N., et al., Mol. Cell. Biol. 21: 5417-5425 (2001). 4. Cotter, M.A., 2nd & Robertson, E.S., Mol. Cell. Biol. 20: 5722-5735 (2000). 5. Di Fiore, B., et al., J. Biol. Chem. 274: 10,339-10,348 (1999). 6. Graziano, V., et al., Nature 368: 351-354 (1994). 7. Hebbes, T.R., et al., EMBO J. 7: 1395-1402 (1988).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,865 Da
NCBI Official Full Name
histone H1.4
NCBI Official Synonym Full Names
histone cluster 1, H1e
NCBI Official Symbol
HIST1H1E
NCBI Official Synonym Symbols
H1E; H1.4; H1F4; H1s-4; dJ221C16.5
NCBI Protein Information
histone H1.4; histone H1b; histone 1, H1e; H1 histone family, member 4
UniProt Protein Name
Histone H1.4
Protein Family
UniProt Gene Name
HIST1H1E
UniProt Synonym Gene Names
H1F4
UniProt Entry Name
H14_HUMAN
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Product Notes
The HIST1H1E hist1h1e (Catalog #AAA6244209) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone H1 (PE) reacts with Bovine, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Western Blot (WB). Researchers should empirically determine the suitability of the HIST1H1E hist1h1e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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