Mouse anti-Human, Rat HIPK2 Monoclonal Antibody | anti-HIPK2 antibody

Mouse Monoclonal [clone 1F10] (IgG2a,k) to Human HIPK2

Cat. Num. AAA246205

• Gene Names: HIPK2; PRO0593
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: HIPK2; Monoclonal Antibody; Mouse Monoclonal [clone 1F10] (IgG2a; k) to Human HIPK2; Anti-HIPK2 Antibody (clone 1F10) IHC-plus; HHIPk2; PRO0593; Stank; Human HIPK2; anti-HIPK2 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1F10
• Specificity: Human HIPK2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1198

• Applicable Applications for anti-HIPK2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate.
• Target Species: Human
• Immunogen Description: HIPK2 (AAG41236, 961 a.a. ~ 1065 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: HIPK2 antibody was raised against hIPK2 (AAG41236, 961 a.a. ~ 1065 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-HIPK2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of HIPK2 expression in RIN-m5F cells with HIPK2 monoclonal antibody, clone 1F10.)

Western Blot (WB)

(Western blot of HIPK2 expression in MES-SA/Dx5 cells with HIPK2 monoclonal antibody, clone 1F10.)

ELISA (EIA)

(Detection limit for recombinant GST tagged HIPK2 is approximately 0.1 ng/ml as a capture antibody.)

Related Product Information for anti-HIPK2 antibody

HIPK2, a nuclear serine/threonine kinase similar to the MNB/DYRK type protein kinases, represses transcription mediated by homeodomain containing transcription factors. HIPK2 is activated by ultraviolet (UV) radiation and selectively phosphorylates p53 to promote p53-dependent gene expression. HIPK2 interacts with various proteins, including members of the p53 family, to regulate cell cycle and apoptosis. Variants of HIPK2 have been observed due to alternative splicing.

Product Categories/Family for anti-HIPK2 antibody

Family: Protein Kinase; Subfamily: MNB/DYRK

NCBI and Uniprot Product Information

• NCBI GI #: 164420685
• NCBI GeneID: 28996
• NCBI Accession #: NP_073577.3
• NCBI GenBank Nucleotide #: NM_022740.4
• UniProt Accession #: Q9H2X6; Q75MR7; Q8WWI4; Q9H2Y1
• Molecular Weight: 128,158 Da
• NCBI Official Full Name: homeodomain-interacting protein kinase 2 isoform 1
• NCBI Official Synonym Full Names: homeodomain interacting protein kinase 2
• NCBI Official Symbol: HIPK2
• NCBI Official Synonym Symbols: PRO0593
• NCBI Protein Information: homeodomain-interacting protein kinase 2; hHIPk2
• UniProt Protein Name: Homeodomain-interacting protein kinase 2
• Protein Family: Homeodomain-interacting protein kinase
• UniProt Gene Name: HIPK2
• UniProt Synonym Gene Names: hHIPk2
• UniProt Entry Name: HIPK2_HUMAN

NCBI Description

This gene encodes a conserved serine/threonine kinase that is a member of the homeodomain-interacting protein kinase family. The encoded protein interacts with homeodomain transcription factors and many other transcription factors such as p53, and can function as both a corepressor and a coactivator depending on the transcription factor and its subcellular localization. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

HIPK2: homeodomain interacting protein kinase 2 is a CMGC kinase of the DYRK family. Interacts with TRADD. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB. Highly expressed in neuronal tissues, heart and kidney. Weakly expressed ubiquitously. Possible tumor suppressor. Activates p53 in response to UV light, causing growth arrest and apoptosis. Required for cisplatin-induced apoptosis. Downregulated in breast and thyroid carcinomas. Three alternatively spliced isoforms have been described.

Protein type: Protein kinase, CMGC; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; DYRK family; HIPK subfamily

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: nuclear body; PML body; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; virion binding; SMAD binding; transcription corepressor activity; ATP binding; protein kinase activity

Biological Process: voluntary musculoskeletal movement; positive regulation of protein binding; regulation of cell cycle; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; PML body organization and biogenesis; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; adult walking behavior; negative regulation of BMP signaling pathway; neuron differentiation; anterior/posterior pattern formation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; erythrocyte differentiation; negative regulation of neuron apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; positive regulation of DNA binding; smoothened signaling pathway; transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; peptidyl-threonine phosphorylation; peptidyl-serine phosphorylation; positive regulation of angiogenesis; eye development; virus-host interaction; embryonic retina morphogenesis in camera-type eye; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; embryonic camera-type eye morphogenesis; positive regulation of protein amino acid phosphorylation