Western Blot (WB)
(Western blot detection of HINT1 in A549,HL-60,U251 cell lysates using HINT1 Rabbit mAb(1:1000 diluted). Predicted band size:14kDa. Observed band size:14kDa.)
Rabbit HINT1 Monoclonal Antibody | anti-HINT1 antibody
HINT1 Rabbit mAb
IHC: 1:50
ICC/IF: 1:50
FC: 1:20-1:100
IP: 1:20
Western Blot (WB)
(Western blot detection of HINT1 in A549,HL-60,U251 cell lysates using HINT1 Rabbit mAb(1:1000 diluted). Predicted band size:14kDa. Observed band size:14kDa.)
Western Blot (WB)
(Western blot detection of HINT1 in A549,HL-60,U251 cell lysates using HINT1 Rabbit mAb(1:1000 diluted). Predicted band size:14kDa. Observed band size:14kDa.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. This gene has been found to be a tumor suppressing gene. Several transcript variants, but only one of them protein-coding, have been found for this gene. [provided by RefSeq, Dec 2012]
Uniprot Description
HINT1: Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha- acetyl lysine methyl ester and AMP-NH2. Belongs to the HINT family.
Protein type: Cytoskeletal; EC 3.-.-.-; Hydrolase
Chromosomal Location of Human Ortholog: 5q31.2
Cellular Component: cytoskeleton; histone deacetylase complex; cytoplasm; plasma membrane; nucleus
Molecular Function: protein kinase C binding; hydrolase activity; nucleotide binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; purine ribonucleotide catabolic process; signal transduction
Disease: Neuromyotonia And Axonal Neuropathy, Autosomal Recessive
Research Articles on HINT1
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Product Notes
The HINT1 hint1 (Catalog #AAA4753298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HINT1 Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HINT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000 IHC: 1:50 ICC/IF: 1:50 FC: 1:20-1:100 IP: 1:20. Researchers should empirically determine the suitability of the HINT1 hint1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HINT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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