Mouse HIF-1 alpha Monoclonal Antibody | anti-HIF-1 alpha antibody
Mouse anti HIF-1 alpha [ESEE122]: Fluorescein
Reactivity
Mouse, Rat, Cow, Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Purified on protein A from tissue culture supernatant.
Synonyms
HIF-1 alpha; Monoclonal Antibody; Mouse anti HIF-1 alpha [ESEE122]: Fluorescein; ARNT interacting protein; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF-1-alpha; HIF-1alpha; HIF1; HIF1-alpha; HIF1A; hypoxia inducible factor 1; alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1; Hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; anti-HIF-1 alpha antibody
Host
Mouse
Reactivity
Mouse, Rat, Cow, Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ESEE122
Specificity
This antibody recognises hypoxia-inducible factor 1 alpha (HIF-1 alpha). Induction of the Hif regulated genes, as a consequence of the microenvironment or genetic changes, is known to have an important role in the growth of experimental tumours. HIF1A has been observed in varying subsets of tumour cells from various solid tumours.
Purity/Purification
Purified on protein A from tissue culture supernatant.
Form/Format
Purified IgG conjugated to Fluorescein, supplied in Phosphate buffered saline (PBS) containing 0.09% Sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-HIF-1 alpha antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Optimal antibody dilution should be determined by titration
Target Antigen
HIF-1 alpha
Immunogen
GST-human HIF-1A amino acids 329-530 fusion protein
Myeloma/fusion Partners
Cells from immunized Balb/c mice were fused with the NS1 myeloma cell line
Research Areas
Epigenetics; DNA binding; DNA damage & repair; Transcription
References
Talks KL, et al. The expression and distribution of the hypoxia-inducible factors HIF-1alpha and HIF-2alpha in normal human tissues, cancers, and tumor-associated macrophages. Am J Pathol.2000.157(2):411-21. PMID:10934146 http://www.ncbi.nlm.nih.gov/pubmed/10934146
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Product Notes
The HIF-1 alpha (Catalog #AAA375368) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti HIF-1 alpha [ESEE122]: Fluorescein reacts with Mouse, Rat, Cow, Human and may cross-react with other species as described in the data sheet. AAA Biotech's HIF-1 alpha can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). Optimal antibody dilution should be determined by titration. Researchers should empirically determine the suitability of the HIF-1 alpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIF-1 alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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