Mouse Helicobacter pylori Urease, Small Subunit Antigen Monoclonal Antibody
Helicobacter pylori Urease, Small Subunit Antigen
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Helicobacter pylori Urease; Small Subunit Antigen; Monoclonal Antibody; Anti -Helicobacter pylori Urease; anti-Helicobacter pylori Urease; Small Subunit Antigen antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5i56
Specificity
Recognizes Helicobacter pylori Urease, small subunit.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-Helicobacter pylori Urease, Small Subunit Antigen antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Dilution:
ELISA: 1:100-1:2500
Western Blot: 1:100-1:2500
Optimal dilutions to be determined by the researcher
ELISA: 1:100-1:2500
Western Blot: 1:100-1:2500
Optimal dilutions to be determined by the researcher
Immunogen
Highly purified H. pylori Urease, small subunit.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Helicobacter pylori Urease, Small Subunit Antigen antibody
H. pylori produces large amounts of the enzyme urease, molecules of which are localized inside and outside of the bacterium. Urease breaks down urea (which is normally secreted into the stomach) to carbon dioxide and ammonia. The ammonia is converted to ammonium by accepting a proton (H+), which neutralizes gastric acid. The survival of H. pylori in the acidic stomach is dependent on urease. The ammonia produced is toxic to the epithelial cells, and along with the other products of H. pylori—including proteases, vacuolating cytotoxin A (VacA), and certain phospholipases, damages those cells.
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Product Notes
The Helicobacter pylori Urease, Small Subunit Antigen (Catalog #AAA603529) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Helicobacter pylori Urease, Small Subunit Antigen can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Dilution: ELISA: 1:100-1:2500 Western Blot: 1:100-1:2500 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Helicobacter pylori Urease, Small Subunit Antigen for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Helicobacter pylori Urease, Small Subunit Antigen, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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