Mouse HDLBP Monoclonal Antibody | anti-HDLBP antibody

HDLBP (High Density Lipoprotein Binding Protein, HDL-Binding Protein, HBP, FLJ16432, PRO2900, Vigilin, VGL)

Cat. Num. AAA603299

• Gene Names: HDLBP; HBP; VGL; PRO2900; FLJ16432
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: HDLBP; Monoclonal Antibody; HDLBP (High Density Lipoprotein Binding Protein; HDL-Binding Protein; HBP; FLJ16432; PRO2900; Vigilin; VGL); Anti -HDLBP (High Density Lipoprotein Binding Protein; anti-HDLBP antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C172
• Specificity: Recognizes human HDLBP.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-HDLBP antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blotting: 1:1000-1:2000
• Immunogen: Partial sequence of recombinant full-length protein to human HDLBP
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Western Blot (WB)

(Detection of HDLBP by Western blot. Samples: Whole cell lysate from human HEK293 (H, 25 μg) , mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. Predicted molecular weight: 141 kD)

Immunostaining (IS)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS603299 mAb.)

Product Categories/Family for anti-HDLBP antibody

Antibodies; Abs to Lipoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 87578140
• NCBI GeneID: 3069
• UniProt Accession #: Q2HJC8; Q00341; Q5HYC5; Q6ZN49; Q6ZS03; Q96CF6; Q9P1C0
• Molecular Weight: 65,353 Da
• NCBI Official Full Name: HDLBP protein
• NCBI Official Synonym Full Names: high density lipoprotein binding protein
• NCBI Official Symbol: HDLBP
• NCBI Official Synonym Symbols: HBP; VGL; PRO2900; FLJ16432
• NCBI Protein Information: vigilin; OTTHUMP00000164572; OTTHUMP00000200288; OTTHUMP00000200289; OTTHUMP00000200290; HDL-binding protein; high density lipoprotein-binding protein
• UniProt Protein Name: HDLBP protein
• Protein Family: Vigilin
• UniProt Gene Name: HDLBP
• UniProt Entry Name: Q2HJC8_HUMAN

NCBI Description

High density lipoprotein-binding protein, also known as vigilin, is a 110-kD protein that specifically binds HDL molecules and may function in the removal of excess cellular cholesterol.[supplied by OMIM]

Uniprot Description

vigilin: a nuclear and cytoplasmic protein that may play a role in cell sterol metabolism. It may function to protect cells from over-accumulation of cholesterol.

Protein type: Lipid-binding; RNA-binding

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; lipid binding

Biological Process: cholesterol metabolic process; lipid transport