Western Blot (WB)
(Anti-DAPK1 Antibody T1316 at 1:4000 dilution + A549 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 160 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Mouse anti-Human, Mouse hDAPK1-T1316 Monoclonal Antibody | anti-hDAPK1-T1316 antibody
hDAPK1-T1316
Gene Names
DAPK1; DAPK
Reactivity
Human, Mouse
Applications
Western Blot
Synonyms
hDAPK1-T1316; Monoclonal Antibody; Purified Mouse Monoclonal Antibody (Mab); Death-associated protein kinase 1; DAP kinase 1; 2.7.11.1; DAPK1; DAPK; anti-hDAPK1-T1316 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a,kappa
Clone Number
2196CT239.5.2
Specificity
This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from human.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applicable Applications for anti-hDAPK1-T1316 antibody
Western Blot (WB)
Application Notes
WB: 1:4000
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Anti-DAPK1 Antibody T1316 at 1:4000 dilution + A549 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 160 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-DAPK1 Antibody T1316 at 1:4000 dilution + A549 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 160 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-hDAPK1-T1316 antibody
Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.
Product Categories/Family for anti-hDAPK1-T1316 antibody
References
Deiss L.P.,et al.Genes Dev. 9:15-30(1995).Feinstein E.,et al.Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.Ota T.,et al.Nat. Genet. 36:40-45(2004).Bechtel S.,et al.BMC Genomics 8:399-399(2007).Humphray S.J.,et al.Nature 429:369-374(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
1430
NCBI Official Full Name
death-associated protein kinase 1
NCBI Official Synonym Full Names
death-associated protein kinase 1
NCBI Official Symbol
DAPK1
NCBI Official Synonym Symbols
DAPK
NCBI Protein Information
death-associated protein kinase 1; DAP kinase 1
UniProt Protein Name
Death-associated protein kinase 1
UniProt Gene Name
DAPK1
UniProt Synonym Gene Names
DAPK; DAP kinase 1
UniProt Entry Name
DAPK1_HUMAN
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Product Notes
The hDAPK1-T1316 dapk1 (Catalog #AAA9232306) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The hDAPK1-T1316 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's hDAPK1-T1316 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:4000. Researchers should empirically determine the suitability of the hDAPK1-T1316 dapk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hDAPK1-T1316, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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