Mouse anti-Mouse, rat HCN2 Cyclic nucleotide-gated Channel Monoclonal Antibody | anti-HCN2 antibody
Anti-HCN2 Cyclic Nucleotide-Gated Channel Monoclonal Antibody
Gene Names
HCN2; BCNG2; HAC-1; BCNG-2
Reactivity
Mouse, rat
Applications
Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
HCN2 Cyclic nucleotide-gated Channel; Monoclonal Antibody; Anti-HCN2 Cyclic Nucleotide-Gated Channel Monoclonal Antibody; anti-HCN2 antibody
Host
Mouse
Reactivity
Mouse, rat
Clonality
Monoclonal
Isotype
lgG1
Clone Number
S71-37
Specificity
HCN2 Cyclic nucleotide-gated Channel
This antibody recognizes mouse and rat HCN2. It does not cross-react with HCN1.
This antibody recognizes mouse and rat HCN2. It does not cross-react with HCN1.
Form/Format
100ug in PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.
Sequence Positions
761-863
Applicable Applications for anti-HCN2 antibody
Immunoblot; Immunohistochemistry; Immunocytochemistry; Immunofluorescence
Application Notes
Immunoblotting: use at 1-10ug/ml. A band of ~95kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml. Immunofluorescence: use at 1-10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate
Antigen
Fusion protein aa 761-863 (cytoplasmic C-terminus) of rat HCN2 (accession number Q9JKA9).
Dilution Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid repeated freezing and thawing.
Related Product Information for anti-HCN2 antibody
Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, play a crucial role in the regulation of cell excitability. They contribute to spontaneous rhythmic activity in the heart and brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
96,950 Da
NCBI Official Full Name
hyperpolarization-activated, cyclic nucleotide-gated channel 2
NCBI Official Synonym Full Names
hyperpolarization activated cyclic nucleotide-gated potassium channel 2
NCBI Official Symbol
HCN2
NCBI Official Synonym Symbols
BCNG2; HAC-1; BCNG-2
NCBI Protein Information
potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; brain cyclic nucleotide gated channel 2; brain cyclic nucleotide-gated channel 2
UniProt Protein Name
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
UniProt Gene Name
HCN2
UniProt Synonym Gene Names
BCNG2
UniProt Entry Name
HCN2_HUMAN
NCBI Description
Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM]
Uniprot Description
HCN2: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. Belongs to the potassium channel HCN family.
Protein type: Membrane protein, multi-pass; Channel, cation; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: voltage-gated potassium channel complex; integral to plasma membrane; plasma membrane
Molecular Function: identical protein binding; voltage-gated potassium channel activity; intracellular cAMP activated cation channel activity; voltage-gated sodium channel activity; cAMP binding
Biological Process: synaptic transmission; regulation of membrane potential; cell-cell signaling
Research Articles on HCN2
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Product Notes
The HCN2 hcn2 (Catalog #AAA190576) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 761-863. The Anti-HCN2 Cyclic Nucleotide-Gated Channel Monoclonal Antibody reacts with Mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's HCN2 Cyclic nucleotide-gated Channel can be used in a range of immunoassay formats including, but not limited to, Immunoblot; Immunohistochemistry; Immunocytochemistry; Immunofluorescence. Immunoblotting: use at 1-10ug/ml. A band of ~95kDa is detected. Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/ml. Immunofluorescence: use at 1-10ug/ml. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate. Researchers should empirically determine the suitability of the HCN2 hcn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HCN2 Cyclic nucleotide-gated Channel, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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