Western Blot (WB)
(Figure 1: Western blot analysis using HAND1 mouse mAb against truncated Trx-HAND1 recombinant protein (1).)
Mouse anti-Human HAND1 Monoclonal Antibody | anti-HAND1 antibody
HAND1 Antibody
Gene Names
HAND1; Hxt; eHand; Thing1; bHLHa27
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-Chromatography
Synonyms
HAND1; Monoclonal Antibody; HAND1 Antibody; autonomic nervous system and neural crest derivatives-expressed protein 1; Basic helix loop helix transcription factor HAND1; bHLHa27; Class A basic helix-loop-helix protein 27; eHAND; Extraembryonic tissues; Extraembryonic tissues heart autonomic nervous system and neural crest derivatives expressed protein 1; HAND 1; HAND1_HUMAN; Heart and neural crest derivatives expressed 1; Heart and neural crest derivatives expressed protein 1; heart; Heart-and neural crest derivatives-expressed protein 1; Hxt; Thing 1; Thing1; anti-HAND1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
HAND1 antibody detects endogenous levels of total HAND1
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
215
Applicable Applications for anti-HAND1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
WB: 1:500-1:2000
Immunogen
Purified recombinant fragment of human HAND1 expressed in E. Coli
Subcellular Location
Nucleus > Nucleoplasm. Nucleus > Nucleolus. Interaction with MDFIC sequesters it into the nucleolus, preventing the transcription factor activity. Phosphorylation by PLK4 disrupts the interaction with MDFIC and releases it from the nucleolus, leading to transcription factor activity.
Tissue Specificity
Heart.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using HAND1 mouse mAb against truncated Trx-HAND1 recombinant protein (1).)
Western Blot (WB)
(Figure 1: Western blot analysis using HAND1 mouse mAb against truncated Trx-HAND1 recombinant protein (1).)
Related Product Information for anti-HAND1 antibody
Description: The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation.
Function: Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) (By similarity).
Subunit Structure: Efficient DNA binding requires dimerization with another bHLH protein. Forms homodimers and heterodimers with TCF3 gene products E12 and E47, HAND2 and HEY1, HEY2 and HEYL (hairy-related transcription factors). Interacts with MDFIC (By similarity).
Post-translational Modifications: Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to translocation into the nucleoplasm, allowing dimerization and transcription factor activity.
Function: Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) (By similarity).
Subunit Structure: Efficient DNA binding requires dimerization with another bHLH protein. Forms homodimers and heterodimers with TCF3 gene products E12 and E47, HAND2 and HEY1, HEY2 and HEYL (hairy-related transcription factors). Interacts with MDFIC (By similarity).
Post-translational Modifications: Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to translocation into the nucleoplasm, allowing dimerization and transcription factor activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 24 kDa
Predicted: 24 kDa
Predicted: 24 kDa
NCBI Official Full Name
heart- and neural crest derivatives-expressed protein 1
NCBI Official Synonym Full Names
heart and neural crest derivatives expressed 1
NCBI Official Symbol
HAND1
NCBI Official Synonym Symbols
Hxt; eHand; Thing1; bHLHa27
NCBI Protein Information
heart- and neural crest derivatives-expressed protein 1
UniProt Protein Name
Heart- and neural crest derivatives-expressed protein 1
Protein Family
UniProt Gene Name
HAND1
UniProt Synonym Gene Names
BHLHA27; EHAND; bHLHa27; eHAND
NCBI Description
The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008]
Uniprot Description
Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) ().
Research Articles on HAND1
Similar Products
Product Notes
The HAND1 hand1 (Catalog #AAA9602510) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HAND1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HAND1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the HAND1 hand1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HAND1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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