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Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: MDA-MB231 cell lysate Lane 2.: WiDr cell lysate Lane 3.: SW480 cell lysate Lane 4.: A427 cell lysate Lane 5.: MCF7 cell lysate Lane 6.: 293T cell lysate Lane 7.: HeLa cell lysate Lane 8.: HT1080 cell lysate Lane 9.: U87MG cell lysate Lane 10.: A549 cell lysate )

Mouse anti-Human GSS Monoclonal Antibody | anti-GSS antibody

GSS antibody

Gene Names
GSS; GSHS; HEL-S-64p; HEL-S-88n
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
GSS; Monoclonal Antibody; GSS antibody; Glutathione synthetase; GSHS; GSH synthetase; anti-GSS antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT12F2
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
474
Applicable Applications for anti-GSS antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human GSS (1-474aa) purified from E. coli
Clone Note
Anti-human GSS mAb, clone AT12F2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GSS protein
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: MDA-MB231 cell lysate Lane 2.: WiDr cell lysate Lane 3.: SW480 cell lysate Lane 4.: A427 cell lysate Lane 5.: MCF7 cell lysate Lane 6.: 293T cell lysate Lane 7.: HeLa cell lysate Lane 8.: HT1080 cell lysate Lane 9.: U87MG cell lysate Lane 10.: A549 cell lysate )

Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: MDA-MB231 cell lysate Lane 2.: WiDr cell lysate Lane 3.: SW480 cell lysate Lane 4.: A427 cell lysate Lane 5.: MCF7 cell lysate Lane 6.: 293T cell lysate Lane 7.: HeLa cell lysate Lane 8.: HT1080 cell lysate Lane 9.: U87MG cell lysate Lane 10.: A549 cell lysate )

Immunofluorescence (IF)

(ICC/IF analysis of GSS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of GSS in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Immunofluorescence (IF) (ICC/IF analysis of GSS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of GSS in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-GSS antibody
Glutathione synthetase, also known GSS, is the second enzyme in the glutathione biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine, to form glutathione. Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency); also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.
Product Categories/Family for anti-GSS antibody
References
Polekhina G., et al. (1999) EMBO J. 18: 3204-3213. ; Huanq Z A., et al. (2000) Biochim Biophys Acta. 1493: 48-55.;

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,349 Da
NCBI Official Full Name
glutathione synthetase
NCBI Official Synonym Full Names
glutathione synthetase
NCBI Official Symbol
GSS
NCBI Official Synonym Symbols
GSHS; HEL-S-64p; HEL-S-88n
NCBI Protein Information
glutathione synthetase
UniProt Protein Name
Glutathione synthetase
Protein Family
UniProt Gene Name
GSS
UniProt Synonym Gene Names
GSH synthetase; GSH-S

NCBI Description

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008]

Research Articles on GSS

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Product Notes

The GSS gss (Catalog #AAA200421) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GSS antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GSS can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the GSS gss for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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