Western Blot (WB)
(Western blot detection of GRB2 in Jurkat,Raji,PC-12&3T3 cell lysates and using GRB2 antibody(1:500 diluted).Predicted band size: 25KDa Observed band size: 25KDa)
Mouse GRB2 Monoclonal Antibody | anti-GRB2 antibody
GRB2 Monoclonal Antibody
Gene Names
GRB2; ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
Reactivity
Human, Rat, Mouse, Monkey
Applications
Western Blot
Synonyms
GRB2; Monoclonal Antibody; GRB2 Monoclonal Antibody; Abundant SRC homology; Adapter protein GRB2; ASH; Ash protein; EGFRBP GRB2; Epidermal growth factor receptor binding protein; Epidermal growth factor receptor binding protein GRB2; GRB 2; GRB2 adapter protein; Grb2; GRB2_HUMAN; Grb3 3; anti-GRB2 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse, Monkey
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3B2-G2-E5
Specificity
This antibody detects endogenous levels of GRB2 and does not cross-react with related proteins.
Form/Format
Ascites
Sequence Length
217
Applicable Applications for anti-GRB2 antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500
Immunogen Type
Recombinant Protein
Immunogen Description
Purified recombinant human GRB2 protein fragments expressed in E Coli.
Target Name
GRB2
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Western blot detection of GRB2 in Jurkat,Raji,PC-12&3T3 cell lysates and using GRB2 antibody(1:500 diluted).Predicted band size: 25KDa Observed band size: 25KDa)
Western Blot (WB)
(Western blot detection of GRB2 in Jurkat,Raji,PC-12&3T3 cell lysates and using GRB2 antibody(1:500 diluted).Predicted band size: 25KDa Observed band size: 25KDa)
Western Blot (WB)
(Western blot detection of GRB2 in MCF7,3T3,COS7,C6 and Hela cell lysates and using GRB2 antibody(1:300 diluted).Predicted band size: 25KDa.Observed band size: 25KDa.)
Western Blot (WB)
(Western blot detection of GRB2 in MCF7,3T3,COS7,C6 and Hela cell lysates and using GRB2 antibody(1:300 diluted).Predicted band size: 25KDa.Observed band size: 25KDa.)
Related Product Information for anti-GRB2 antibody
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Isoform 2 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform 2 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
Product Categories/Family for anti-GRB2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,557 Da
NCBI Official Full Name
growth factor receptor-bound protein 2 isoform 1
NCBI Official Synonym Full Names
growth factor receptor-bound protein 2
NCBI Official Symbol
GRB2
NCBI Official Synonym Symbols
ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
NCBI Protein Information
growth factor receptor-bound protein 2
UniProt Protein Name
Growth factor receptor-bound protein 2
Protein Family
UniProt Gene Name
GRB2
UniProt Synonym Gene Names
ASH
UniProt Entry Name
GRB2_HUMAN
NCBI Description
The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Research Articles on GRB2
Similar Products
Product Notes
The GRB2 grb2 (Catalog #AAA9400488) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GRB2 Monoclonal Antibody reacts with Human, Rat, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's GRB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500. Researchers should empirically determine the suitability of the GRB2 grb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GRB2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
