Mouse anti-Human GPX1 Monoclonal Antibody | anti-GPX1 antibody

GPX1 Monoclonal Antibody

Cat. Num. AAA3014471

• Gene Names: GPX1; GPXD; GSHPX1
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: GPX1; Monoclonal Antibody; GPX1 Monoclonal Antibody; GSHPx-1; GPx-1; AL033363; Cellular glutathione peroxidase; Glutathione peroxidase 1; Glutathione peroxidase; GPx 1; GPXD; GSHPX1; MGC14399; MGC88245; anti-GPX1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Specificity: GPX1 monoclonal antibody detects endogenous levels of GPX1 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Mouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 203

• Applicable Applications for anti-GPX1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1,000; IHC: 1:200; ICC: 1:200; FC/FACS: 1:100-1:200
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Positive control: Western Blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1000 dilution. Positive control: Lane 1: THP-1 Lane 2: HepG2 Lane 3: 293T)

Immunocytochemistry (ICC)

(ICC staining GPX1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-GPX1 antibody

GPX1 is ubiquitously expressed in many tissues, where it protects cells from oxidative stress. Within cells, it localizes to the cytoplasm and mitochondria. As a glutathione peroxidase, GPx1 functions in the detoxification of hydrogen peroxide, specifically by catalyzing the reduction of hydrogen peroxide to water. The glutathione peroxidase also catalyzes the reduction of other organic hydroperoxides, such as lipid peroxides, to the corresponding alcohols. GPx1 typically uses glutathione (GSH) as the reductant, but when glutathione synthetase (GSS) is, as in brain mitochondria, gamma-glutamylcysteine can serve as the reductant instead. The protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell death and increases resistance to toxic challenges, especially oxidative stress. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.

NCBI and Uniprot Product Information

• NCBI GI #: 41406084
• NCBI GeneID: 2876
• NCBI Accession #: NP_000572.2
• NCBI GenBank Nucleotide #: NP_000572.2
• UniProt Accession #: P07203
• Molecular Weight: 22kDa
• NCBI Official Full Name: glutathione peroxidase 1 isoform 1
• NCBI Official Synonym Full Names: glutathione peroxidase 1
• NCBI Official Symbol: GPX1
• NCBI Official Synonym Symbols: GPXD; GSHPX1
• NCBI Protein Information: glutathione peroxidase 1
• UniProt Protein Name: Glutathione peroxidase 1
• Protein Family: Hydroperoxy fatty acid reductase
• UniProt Gene Name: GPX1
• UniProt Synonym Gene Names: GPx-1; GSHPx-1
• UniProt Entry Name: GPX1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Other studies indicate that H2O2 is also essential for growth-factor mediated signal transduction, mitochondrial function, and maintenance of thiol redox-balance; therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is the most abundant, is ubiquitously expressed and localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. It is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. This gene contains an in-frame GCG trinucleotide repeat in the coding region, and three alleles with 4, 5 or 6 repeats have been found in the human population. The allele with 4 GCG repeats has been significantly associated with breast cancer risk in premenopausal women. Alternatively spliced transcript variants have been found for this gene. Pseudogenes of this locus have been identified on chromosomes X and 21. [provided by RefSeq, Aug 2017]

Uniprot Description

GPX1: Protects the hemoglobin in erythrocytes from oxidative breakdown. Homotetramer. Interacts with MIEN1. Belongs to the glutathione peroxidase family.

Protein type: Other Amino Acids Metabolism - glutathione; EC 1.11.1.9; Lipid Metabolism - arachidonic acid; Oxidoreductase

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nucleus; cytosol

Molecular Function: selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity; SH3 domain binding

Biological Process: response to nicotine; fat cell differentiation; blood vessel endothelial cell migration; myoblast proliferation; response to toxin; skeletal muscle fiber development; negative regulation of caspase activity; thermoregulation; vasodilation; regulation of gene expression, epigenetic; endothelial cell development; response to estradiol stimulus; regulation of mammary gland epithelial cell proliferation; response to selenium ion; glutathione metabolic process; sensory perception of sound; lipoxygenase pathway; UV protection; response to symbiotic bacterium; hydrogen peroxide catabolic process; response to folic acid; regulation of neuron apoptosis; response to glucose stimulus; response to gamma radiation; arachidonic acid metabolic process; negative regulation of inflammatory response to antigenic stimulus; aging; protein amino acid oxidation; nucleobase, nucleoside and nucleotide metabolic process; skeletal muscle regeneration; angiogenesis involved in wound healing; purine base metabolic process; positive regulation of protein kinase B signaling cascade; response to xenobiotic stimulus; response to reactive oxygen species; triacylglycerol metabolic process; response to hydrogen peroxide; cell redox homeostasis; response to lipid hydroperoxide; heart contraction; purine nucleotide catabolic process; interaction with symbiont; induction of apoptosis by oxidative stress

Disease: Glutathione Peroxidase Deficiency