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Western Blot (WB) ()

Mouse GPI PLD Monoclonal Antibody | anti-GPI PLD antibody

GPI PLD antibody

Gene Names
Gpld1; AW546131; 6330541J12Rik
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
GPI PLD antibody was purified by ammonium sulfate precipitation
Synonyms
GPI PLD; Monoclonal Antibody; GPI PLD antibody; Monoclonal GPI PLD; Anti-GPI PLD; GPLD1; glycosylphosphatidylinositol specific phospholipase D1; BOS_21856; glycoprotein phospholipase D; glycosylphosphatidylinositol phospholipase D; glycosyl-phosphatidylinositol-specific phospholipase D; GPIPLD; GPI-PLD; GPIPLDM; GPI-specific phospholipase D; phosphatidylinositol-glycan-specific phospholipase D; phosphatidylinositol-glycan-specific phospholipase D-like; PIGPLD; PI-G PLD; PIGPLD1; RP23-123C5.1; anti-GPI PLD antibody
Ordering
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
38A1
Purity/Purification
GPI PLD antibody was purified by ammonium sulfate precipitation
Form/Format
Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol
Sequence Length
842
Applicable Applications for anti-GPI PLD antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
WB: 1:500
IHC-P
Biological Significance
GPI-PLD (glycosylphosphati- dylinositol-specific phospholipase D), a 815-amino acid protein, is expressed in numerous tissues and cells and specifically cleaves GPI-anchored proteins. Liver has the highest level of GPI-PLD expression and is the primary organ contributing to GPI-PLD in the serum. GPI-PLD is abundant in serum in which it associates with apolipoproteins AI and AIV. Increased serum GPI-PLD is associated with insulin resistance and elevated serum triglycerides. Many surface proteins are attached to eukaryotic cell membranes via glycosylphosphatidylinositol (GPI) anchors that are covalently bound to the C-terminus of the protein and cleavage of the GPI moiety by GPI-PLD, only enzyme known that cleavage GPI anchor, may represent a means of regulating attachment of these proteins to the cell surface, or alternatively, their release into the extracellular environment.
Cross-Reactivity
Human
Immunogen
GPI PLD antibody was raised in Mouse using a protein purified from Human plasma as the immunogen
Preparation and Storage
Aliquot and store for 1 year at -20 degree C, avoid freeze/thaw cycles

Western Blot (WB)

()

Western Blot (WB) ()
Related Product Information for anti-GPI PLD antibody
Mouse monoclonal GPI PLD antibody
Product Categories/Family for anti-GPI PLD antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
93,255 Da
NCBI Official Full Name
glycosylphosphatidylinositol phospholipase D
NCBI Official Synonym Full Names
glycosylphosphatidylinositol specific phospholipase D1
NCBI Official Symbol
Gpld1
NCBI Official Synonym Symbols
AW546131; 6330541J12Rik
NCBI Protein Information
phosphatidylinositol-glycan-specific phospholipase D
UniProt Protein Name
Phosphatidylinositol-glycan-specific phospholipase D
UniProt Gene Name
Gpld1
UniProt Synonym Gene Names
PI-G PLD; GPI-PLD; GPI-specific phospholipase D
UniProt Entry Name
PHLD_MOUSE

Uniprot Description

GPLD1: This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. Belongs to the GPLD1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Secreted; EC 3.1.4.50; Phospholipase; Secreted, signal peptide

Cellular Component: extracellular space; proteinaceous extracellular matrix; intracellular membrane-bound organelle; lysosomal membrane; cytoplasm; extracellular region; intracellular

Molecular Function: protein binding; sodium channel regulator activity; glycosylphosphatidylinositol phospholipase D activity; hydrolase activity; phospholipase D activity

Biological Process: negative regulation of cell proliferation; cell migration during sprouting angiogenesis; GPI anchor release; cellular response to insulin stimulus; positive regulation of apoptosis; complement receptor mediated signaling pathway; response to glucose stimulus; insulin receptor signaling pathway; positive regulation of secretion; positive regulation of cytolysis; positive regulation of membrane protein ectodomain proteolysis

Research Articles on GPI PLD

Similar Products

Product Notes

The GPI PLD gpld1 (Catalog #AAA834378) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GPI PLD can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: 1:500 IHC-P. Researchers should empirically determine the suitability of the GPI PLD gpld1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPI PLD, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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