Mouse anti-Human, Cow Glycophorin A /CD235a Monoclonal Antibody | anti-GYPA antibody

Glycophorin A /CD235a (Erythrocyte Marker) Mouse Monoclonal Antibody

Cat. Num. AAA438922

• Gene Names: GYPA; MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
• Reactivity: Human, Cow
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: Glycophorin A /CD235a; Monoclonal Antibody; Glycophorin A /CD235a (Erythrocyte Marker) Mouse Monoclonal Antibody; Blood group--MN locus; GPA; GPErik; GpMiIII; GPSAT; GYPA; MN sialoglycoprotein; MNS; PAS2; Sialoglycoprotein alpha; anti-GYPA antibody

• Host: Mouse
• Reactivity: Human, Cow
• Clonality: Monoclonal
• Isotype: IgM, kappa
• Clone Number: SPM183
• Specificity: Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A. It reacts with a peptide epitope on the extracellular domain of human glycophorin. Glycophorin A is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorins acts as the receptor for Sandei virus and parvovirus.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 117

• Applicable Applications for anti-GYPA antibody: Erythrocyte Agglutination, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Erythrocyte Agglutination; Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml)
• Cellular Localization: Cell Surface
• Immunogen: Human erythrocytes treated with neuraminidase
• Hu-Chromosome Location: 4q31.22
• Positive Control: Erythrocytes
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Cartron JP and Rahuel C. Human erythrocyte glycophorins: protein and gene structure analyses. Transfus Med Rev 1992,6(2):63-92 Gahmberg CG et al. Biosynthesis of the major human red cell sialoglycoprotein, glycophorin A. A review. Rev Fr Transfus Immunohematol 1981,24(1):53-73 Wybenga LE et al. Glycophorin as a receptor for Sendai virus. Biochemistry 1996,35(29):9513-8 Rahuel C et al. Post-transcriptional regulation of the cell surface expression of glycophorins A, B, and E. J Biol Chem 1994, 269(52):32752-8 Thacker TC and Johnson FB. Binding of bovine parvovirus to erythrocyte membrane sialylglycoproteins. J Gen Virol 1998, 79:2163-

NCBI and Uniprot Product Information

• NCBI GI #: 815891063
• NCBI GeneID: 2993
• NCBI Accession #: NP_001295119.1
• NCBI GenBank Nucleotide #: NM_001308190.1
• UniProt Accession #: P02724; Q9BS51; A8K3E6; B8Q182; B8Q185
• Molecular Weight: 39kDa
• NCBI Official Full Name: glycophorin-A isoform 3
• NCBI Official Synonym Full Names: glycophorin A (MNS blood group)
• NCBI Official Symbol: GYPA
• NCBI Official Synonym Symbols: MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
• NCBI Protein Information: glycophorin-A
• UniProt Protein Name: Glycophorin-A
• Protein Family: Glycophorin
• UniProt Gene Name: GYPA
• UniProt Synonym Gene Names: GPA
• UniProt Entry Name: GLPA_HUMAN

NCBI Description

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]

Uniprot Description

Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).