Glyceraldehyde-3-Phosphate Dehydrogenase (multiple species) (GAPDH) Monoclonal Antibody | anti-GAPDH antibody

MAb to Glyceraldehyde-3-PDH

Cat. Num. AAA311206

• Gene Names: GAPDH; G3PD; GAPD; MGC88685
• Applications: ELISA, Western Blot
• Purity: >90% pure (SDS-PAGE). Protein A chromatography
• Synonyms: Glyceraldehyde-3-Phosphate Dehydrogenase (multiple species) (GAPDH); Monoclonal Antibody; MAb to Glyceraldehyde-3-PDH; Monoclonal Antibody to Glyceraldehyde-3-PDH (GAPDH); anti-GAPDH antibody

• Host: Mouse. Hybridization of Sp2/0 myeloma cells with spleen cells from BALB/c mice.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6C5
• Specificity: Glyceraldehyde-3-Phosphate Dehydrogenase (multiple species) (GAPDH); Recognizes GAPDH monomer (36kDa) and the dimer form. Does not react with the tetrameric form. Reacts with GAPDH from fish, frog, chicken, rabbit, mouse and human skeletal muscle. Does
• Purity/Purification: >90% pure (SDS-PAGE). Protein A chromatography
• Form/Format: Purified, Liquid
• Concentration: 4mg/ml (Sigma protein assay kit) (varies by lot)
• Sequence Length: 335

• Applicable Applications for anti-GAPDH antibody: EIA/ELISA, Immunoassay Antibody Pairs, Western Blot
• Immunogen: Purified rabbit muscle GAPDH (whole molecule)
• Buffer: PBS, pH 7.4
• Preservative: 0.1% Sodium azide
• Important Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
• Preparation and Storage: Store at 2 to 8 degree C.

Product Categories/Family for anti-GAPDH antibody

Monoclonal Antibodies to Enzymes

References

• Vozenin-Brotons, M.C., et al., (2003), "Fibrogenic signals in patients with radiation enteritis are associated with increased connective tissue growth factor expression", Int. J. Radiation Oncology Biol. Phys., 56(2): 561-572.
• K

NCBI and Uniprot Product Information

• NCBI GI #: 7669492
• NCBI GeneID: 2597
• NCBI Accession #: NP_002037.2
• NCBI GenBank Nucleotide #: NM_002046.3
• UniProt Accession #: P04406; P00354; Q53X65; Q0QET7; Q16768; Q2TSD0; Q5ZEY3
• Molecular Weight: 36,053 Da
• NCBI Official Full Name: glyceraldehyde-3-phosphate dehydrogenase
• NCBI Official Synonym Full Names: glyceraldehyde-3-phosphate dehydrogenase
• NCBI Official Symbol: GAPDH
• NCBI Official Synonym Symbols: G3PD; GAPD; MGC88685
• NCBI Protein Information: glyceraldehyde-3-phosphate dehydrogenase; OTTHUMP00000174430; OTTHUMP00000174431; OTTHUMP00000174432; OTTHUMP00000174434; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase
• UniProt Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• UniProt Gene Name: GAPDH
• UniProt Synonym Gene Names: GAPD
• UniProt Entry Name: G3P_HUMAN

NCBI Description

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

Uniprot Description

Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC

By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Ref.6 Ref.23

Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. Ref.6

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Subunit structure: Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation

By similarity. Interacts with EIF1AD, USP25, PRKCI and WARS. Ref.23 Ref.25 Ref.28 Ref.36 Ref.39 Ref.40

Subcellular location: Cytoplasm › cytosol. Nucleus

By similarity. Cytoplasm › perinuclear region. Membrane. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal

By similarity. Postnuclear and Perinuclear regions. Ref.24

Post-translational modification: S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus

By similarity.ISGylated

Probable. Ref.29

Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.