Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Monoclonal Antibody | anti-GAPDH antibody
MAb to GAPDH
Source: Ascites
Recognizes GAPDH monomer (36kDa) and the dimer form. Does not react with the tetrameric form.
NCBI and Uniprot Product Information
NCBI Description
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]
Uniprot Description
Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC
By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Ref.6 Ref.23
Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. Ref.6
Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure: Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation
By similarity. Interacts with EIF1AD, USP25, PRKCI and WARS. Ref.23 Ref.25 Ref.28 Ref.36 Ref.39 Ref.40
Subcellular location: Cytoplasm › cytosol. Nucleus
By similarity. Cytoplasm › perinuclear region. Membrane. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal
By similarity. Postnuclear and Perinuclear regions. Ref.24
Post-translational modification: S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus
By similarity.ISGylated
Probable. Ref.29
Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Research Articles on GAPDH
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Product Notes
The GAPDH gapdh (Catalog #AAA312511) is an Antibody produced from Host: Mouse. Hybridization of Sp2/0 myeloma cells with spleen cells from BALB/c mice. Source: Ascites and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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