Mouse anti-Human Glutamine Synthetase Monoclonal Antibody | anti-GLUL antibody

Glutamine Synthetase (GS, Glutamate Decarboxylase, Glutamate-ammonia Ligase, GLUL, GLNS)

Cat. Num. AAA633286

• Gene Names: GLUL; GS; GLNS; PIG43; PIG59
• Reactivity: Human
• Applications: Immunoprecipitation
• Purity: Ascites; Ascites
• Synonyms: Glutamine Synthetase; Monoclonal Antibody; Glutamine Synthetase (GS; Glutamate Decarboxylase; Glutamate-ammonia Ligase; GLUL; GLNS); Anti -Glutamine Synthetase (GS; anti-GLUL antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, k
• Clone Number: 10K208
• Specificity: Recognizes human Glutamine Synthetase.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid in Hepes, 0.15M sodium chloride, 0.01% BSA, 0.03% sodium azide, 50% glycerol.

• Applicable Applications for anti-GLUL antibody: Immunoprecipitation (IP)
• Application Notes: Suitable for use in Immunoprecipitation.; Dilution: Immunoprecipitation: 1-2ul/400ul lysates
• Immunogen: Recombinant human protein purified from E.coli.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-GLUL antibody

Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. This reaction consumes a molecule of ATP:Glutamate + NH4+ + ATP, and produces, Glutamine + ADP + Pi. It is found in astrocytes as an octamer of identical 45kD subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in controlling metabolic regulations of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. On the other hands, the level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.

Product Categories/Family for anti-GLUL antibody

Antibodies; Abs to Enzymes; Decarboxylase

NCBI and Uniprot Product Information

• NCBI GI #: 74271837
• NCBI GeneID: 2752
• NCBI Accession #: NP_001028216.1
• NCBI GenBank Nucleotide #: NM_001033044.2
• UniProt Accession #: P15104; Q499Y9; Q5T9Z1; Q7Z3W4; Q8IZ17; Q2TU80; Q9NSP3
• Molecular Weight: 42,064 Da
• NCBI Official Full Name: glutamine synthetase
• NCBI Official Synonym Full Names: glutamate-ammonia ligase
• NCBI Official Symbol: GLUL
• NCBI Official Synonym Symbols: GS; GLNS; PIG43; PIG59
• NCBI Protein Information: glutamine synthetase; OTTHUMP00000035524; OTTHUMP00000035525; glutamine synthase; glutamate decarboxylase; glutamate--ammonia ligase; proliferation-inducing protein 43; cell proliferation-inducing protein 59
• UniProt Protein Name: Glutamine synthetase
• Protein Family: Glutamine synthetase
• UniProt Gene Name: GLUL
• UniProt Synonym Gene Names: GLNS; GS
• UniProt Entry Name: GLNA_HUMAN

NCBI Description

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. This gene is expressed during early fetal stages, and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in this gene are associated with congenital glutamine deficiency. Several alternatively spliced transcript variants have been found for this gene.

Uniprot Description

GLUL: This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner. Essential for proliferation of fetal skin fibroblasts. Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD). CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. Belongs to the glutamine synthetase family.

Protein type: EC 6.3.1.2; Ligase; EC 4.1.1.15; Amino Acid Metabolism - alanine, aspartate and glutamate; Energy Metabolism - nitrogen; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: protein complex; mitochondrion; rough endoplasmic reticulum; cytoplasm; perikaryon; nerve terminal; cytosol; nucleus

Molecular Function: glutamate-ammonia ligase activity; identical protein binding; dynein light chain binding; glutamate binding; glutamate decarboxylase activity; manganese ion binding; magnesium ion binding; ATP binding

Biological Process: cell proliferation; glutamate catabolic process; synaptic transmission; glutamine biosynthetic process; response to glucose stimulus; positive regulation of insulin secretion; neurotransmitter uptake; amino acid biosynthetic process; cellular response to starvation; positive regulation of synaptic transmission, glutamatergic; positive regulation of epithelial cell proliferation; protein homooligomerization

Disease: Glutamine Deficiency, Congenital