Mouse anti-Human Glutamate-Cysteine Ligase Monoclonal Antibody | anti-GCLM antibody

Glutamate-Cysteine Ligase Regulatory Subunit

Cat. Num. AAA4381540

• Gene Names: GCLM; GLCLR
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Glutamate-Cysteine Ligase; Monoclonal Antibody; Glutamate-Cysteine Ligase Regulatory Subunit; GCLM Antibody; Gamma-ECS regulatory subunit Antibody; GLCLR Antibody; GCS light chain Antibody; GSC light chain Antibody; anti-GCLM antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: CPTC-GCLM-1
• Specificity: GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 274

• Applicable Applications for anti-GCLM antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: WB: 1-2ug/ml; IHC-P: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degree C followed by cooling at RT for 20 minutes
• Immunogen: Recombinant Human full-length GCLM protein
• Cellular Localization: Cytoplasm
• Hu-chromosome Location: 1p22.1
• Positive Control: A549, HepG2 or HeLa cell lysates. Human muscle and Liver tissue.
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1). Confirmation of Integrity and Purity of Antibody.)

Western Blot (WB)

(Western Blot Analysis of A549, A431 & HepG2 cell lysates using GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).)

Immunohistochemistry (IHC)-Formalin

(Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).)

References

Tu Z, et al. Arch Biochem Biophys 1998;354:247 254.

NCBI and Uniprot Product Information

• NCBI GI #: 4504011
• NCBI GeneID: 2730
• NCBI Accession #: NP_002052.1
• NCBI GenBank Nucleotide #: NP_002052.1
• UniProt Accession #: P48507
• Molecular Weight: 31kDa
• NCBI Official Full Name: glutamate--cysteine ligase regulatory subunit isoform 1
• NCBI Official Synonym Full Names: glutamate-cysteine ligase modifier subunit
• NCBI Official Symbol: GCLM
• NCBI Official Synonym Symbols: GLCLR
• NCBI Protein Information: glutamate--cysteine ligase regulatory subunit
• UniProt Protein Name: Glutamate--cysteine ligase regulatory subunit
• Protein Family: Glutamate--cysteine ligase regulatory
• UniProt Gene Name: GCLM
• UniProt Synonym Gene Names: GLCLR
• UniProt Entry Name: GSH0_HUMAN

NCBI Description

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Uniprot Description

GCLM: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008]

Protein type: Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: glutamate-cysteine ligase complex; cytosol

Molecular Function: protein heterodimerization activity; glutamate-cysteine ligase activity; enzyme regulator activity; glutamate-cysteine ligase catalytic subunit binding

Biological Process: response to drug; response to nitrosative stress; cysteine metabolic process; apoptotic mitochondrial changes; glutamate metabolic process; sulfur amino acid metabolic process; xenobiotic metabolic process; glutathione biosynthetic process; response to oxidative stress; negative regulation of neuron apoptosis; regulation of mitochondrial depolarization; regulation of blood vessel size; positive regulation of glutamate-cysteine ligase activity

Disease: Myocardial Infarction, Susceptibility To