Mouse anti-Mouse GLUCOCORTICOID RECEPTOR Monoclonal Antibody | anti-NR3C1 antibody

MOUSE ANTI HUMAN GLUCOCORTICOID RECEPTOR:FITC

Cat. Num. AAA210980

• Gene Names: NR3C1; GR; GCR; GRL; GCCR; GCRST
• Reactivity: Mouse
• Applications: Flow Cytometry
• Purity: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
• Synonyms: GLUCOCORTICOID RECEPTOR; Monoclonal Antibody; MOUSE ANTI HUMAN GLUCOCORTICOID RECEPTOR:FITC; anti-NR3C1 antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5E4
• Purity/Purification: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC)-Liquid; Phosphate buffered saline
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 777

• Applicable Applications for anti-NR3C1 antibody: Flow cytometry (FC)
• Application Notes: Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10; Flow Cytometry: Use 10ul of the suggested working dilution to label 1X10⁶ cells in 100ul.; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.; ; Species Cross Reactivity Note: N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories , peer-reviewed publications or personal communications from the originators.
• Perservative Stabilisers: <0.01% Sodium Azide (NaN₃); 1% Bovine Serum Albumin; Preparation
• Immunogen: 26 amino acid peptide corresponding to residues 150-176 on human GCR linked to thyroglobulin.; Fusion Partners
• Target Species: Human
• Max Ex/Em: Fluorophore : FITC; Excitation Max (nm): 490; Emission Max (nm): 525
• Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp-2/0 Ag14 myeloma cell line.
• Preparation and Storage: The product is shipped at ambient tempeture. It is recommended to aliquot and store at -20°C on receipt. When thawed , aliquot the smaple as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks)and store the remaining aliquots at -20°C. ; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Related Product Information for anti-NR3C1 antibody

Mouse anti Human Glucocorticoid Receptor antibody, clone 5E4 recognizes the human glucocorticoid receptor, also known as Nuclear receptor subfamily 3 group C member 1 (NR3C1), a 777 amino acid receptor bearing 3 distinct functional domains, an N-terminal modulating domain, a DNA binding domain and a C-terminal steroid binding domain. The human glucocorticoid receptor is located either in the cytoplasm of cells, prior to ligand binding associated with certain heat shock proteins (Kino et al. 2009), or the nucleus following binding to ligand (Werb et al. 1978). Mutations in the glucocorticoid receptor gene can lead to familial glucocorticoid resistance, characterized by elevated plasma cortisol levels (Malchoff et al. 1993). Mouse anti human glucocorticoid receptor antibody, clone 5E4 has been demonstrated to cross-react with the murine glucocorticoid receptor (Bergquist et al. 2014)

NCBI and Uniprot Product Information

• NCBI GI #: 4504133
• NCBI GeneID: 2908
• NCBI Accession #: NP_000167.1
• NCBI GenBank Nucleotide #: NM_000176.2
• UniProt Accession #: P04150; P04151; Q53EP5; Q6N0A4; A0ZXF9; B0LPG8; D3DQF4
• NCBI Official Full Name: glucocorticoid receptor isoform alpha
• NCBI Official Synonym Full Names: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• NCBI Official Symbol: NR3C1
• NCBI Official Synonym Symbols: GR; GCR; GRL; GCCR; GCRST
• NCBI Protein Information: glucocorticoid receptor; glucocorticoid nuclear receptor variant 1
• UniProt Protein Name: Glucocorticoid receptor
• Protein Family: Glucocorticoid receptor
• UniProt Gene Name: NR3C1
• UniProt Synonym Gene Names: GRL; GR
• UniProt Entry Name: GCR_HUMAN

NCBI Description

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

Uniprot Description

GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described.

Protein type: Transcription factor; Mitochondrial; DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: nucleoplasm; membrane; mitochondrial matrix; cytoplasm; cytosol; nucleus

Molecular Function: protein dimerization activity; glucocorticoid receptor activity; protein binding; zinc ion binding; transcription factor activity; steroid binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; maternal behavior; adrenal gland development; regulation of glucocorticoid biosynthetic process; chromatin modification; signal transduction; glucocorticoid metabolic process; glucocorticoid mediated signaling; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; glucocorticoid receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of gluconeogenesis

Disease: Glucocorticoid Resistance, Generalized