Mouse anti-Human Glucagon-like Peptide 1 Monoclonal Antibody | anti-GCG antibody

Glucagon-like Peptide 1 (GLP1)

Cat. Num. AAA600882

• Gene Names: GCG; GLP1; GLP2; GRPP
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Glucagon-like Peptide 1; Monoclonal Antibody; Glucagon-like Peptide 1 (GLP1); Anti -Glucagon-like Peptide 1 (GLP1); anti-GCG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2B2.6
• Specificity: Recognizes human GLP1 (7-36), GLP1 (1-30), C-terminal specific. Does not crossreact with GLP2, GRPP, PYY, VIP, PHI, GIP, Glucagon or Oxyntomodulin. The stated amino acid sequence for the immunogen is found entirely intact in mouse GLP1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 10ul sterile ddH2O.

• Applicable Applications for anti-GCG antibody: Immunohistochemistry (IHC), Immunocytochemistry (ICC), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in Dot Blot, Immunocytochemistry and Immunohistochemistry.; Dilution: Dot Blot: 1:5000; Immunocytochemistry: 1:10,000; Immunohistochemistry: Paraffin embedded sections
• Immunogen: Synthetic peptide corresponding to aa7-36 amide of human Glucagon Like Peptide 1, conjugated to KLH.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-GCG antibody

GLP1 is a processed active peptide of 26aa (chr 2q36-q37). It is a potent insulin secretagogue and plays a major role in the enteroinsular axis, accounting for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously. Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide

Product Categories/Family for anti-GCG antibody

Antibodies; Abs to Hormones; Steroids

NCBI and Uniprot Product Information

• NCBI GI #: 4503945
• NCBI GeneID: 2641
• NCBI Accession #: NP_002045.1
• NCBI GenBank Nucleotide #: NM_002054.4
• UniProt Accession #: P01275; Q53TP6; A6NN65
• Molecular Weight: 20,909 Da
• NCBI Official Full Name: glucagon preproprotein
• NCBI Official Synonym Full Names: glucagon
• NCBI Official Symbol: GCG
• NCBI Official Synonym Symbols: GLP1; GLP2; GRPP
• NCBI Protein Information: glucagon; preproglucagon; glucagon-like peptide 1; glucagon-like peptide 2; glicentin-related polypeptide
• UniProt Protein Name: Glucagon
• Protein Family: Glucagon
• UniProt Gene Name: GCG
• UniProt Synonym Gene Names: GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2
• UniProt Entry Name: GLUC_HUMAN

NCBI Description

The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008]

Uniprot Description

GCG: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. GLP-1 and GLP-2 are induced in response to nutrient ingestion. Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. Belongs to the glucagon family.

Protein type: Secreted, signal peptide; Hormone; Secreted

Chromosomal Location of Human Ortholog: 2q36-q37

Cellular Component: extracellular space; endoplasmic reticulum lumen; plasma membrane; extracellular region

Molecular Function: identical protein binding; protein binding; hormone activity; glucagon receptor binding; receptor binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; response to starvation; cell proliferation; G-protein coupled receptor protein signaling pathway; cellular protein metabolic process; positive regulation of protein binding; positive regulation of protein kinase activity; positive regulation of cAMP biosynthetic process; energy reserve metabolic process; feeding behavior; positive regulation of histone H3-K4 methylation; signal transduction; positive regulation of peptidyl-serine phosphorylation; regulation of insulin secretion; negative regulation of apoptosis