Mouse anti-Human Glial fibrillary acidic protein / GFAP Monoclonal Antibody | anti-GFAP antibody
Mouse anti Glial fibrillary acidic protein / GFAP
Gene Names
GFAP; FLJ45472; GFAP
Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry, Western Blot
Synonyms
Glial fibrillary acidic protein / GFAP; Monoclonal Antibody; Mouse anti Glial fibrillary acidic protein / GFAP; anti-GFAP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6F2
Specificity
6F2 reacts exclusively with glial fibrillary acidic protein which is present in astrocytes in the central nervous system and Schwann cells.
Form/Format
The vial contains 100 ul 1 mg/ml monoclonal purified antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-GFAP antibody
Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
Application Notes
6F2 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
Source Note
6F2 is a Mouse monoclonal IgG1 antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with glial fibrillary acidic protein from Human brain.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Related Product Information for anti-GFAP antibody
GFAP (55 kD) is selectively loCated in astrocytes and represents the major constituent of astrocytic intermediate filaments. GFAP expression levels are highly variable during development of the central nervous system. In adults, GFAP levels increase as a result of the prolifeRation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer's disease, epilepsy and multiple sclerosis. In the peripheral nervous system GFAP is expressed by Schwann cells. Upon differentiation, myelin forming Schwann cells down-regulate GFAP, whereas in non-myelin forming Schwann cells GFAP persists into adulthood.
Product Categories/Family for anti-GFAP antibody
References
Van Muijen, G. N., Ruiter, D. J., and Warnaar, S. O. (1987). Coexpression of intermediate filament polypeptides in Human fetal and adult tissues, Lab Invest 57, 359-69.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
49,880 Da
NCBI Official Full Name
glial fibrillary acidic protein
NCBI Official Synonym Full Names
glial fibrillary acidic protein
NCBI Official Symbol
GFAP
NCBI Official Synonym Symbols
FLJ45472; GFAP
NCBI Protein Information
glial fibrillary acidic protein
UniProt Protein Name
Glial fibrillary acidic protein
Protein Family
UniProt Gene Name
GFAP
UniProt Entry Name
GFAP_HUMAN
NCBI Description
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]
Uniprot Description
GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: membrane; cytoplasm; intermediate filament; cytosol
Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding
Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development
Disease: Alexander Disease
Research Articles on GFAP
1. Data demonstrate that withaferin A covalently binds soluble recombinant tetrameric human GFAP at cysteine 294.
2. GFAP+1 expressing astrocytes may have a specific role in Alzheimer's disease
3. Results identified glial fibrillary acidic protein that showed elevated levels of protein carbonyls in inferior parietal lobule (IPL) from subjects with early stage-Alzheimer's disease.
4. Determination of relative levels of MBP and GFAP mRNAs may be useful for glial tumors recognition.
5. GFAP-delta may be a useful diagnostic marker for the evaluation of functional cataplasia or proliferation of astrocytic tumor.
6. GFAP is a potential marker for tumors with cartilaginous differentiation.
7. Novel variant (S398F) in the glial fibrillary acidic protein gene is found in a patient with Alexander disease.
8. GFAP-delta is expressed in a variety of pathologies associated with epilepsy, increased expression is shown in regions of astrocytic gliosis
9. GFAP had higher mean expression levels across regions in all psychiatric groups relative to normal control.
10. the best immunohistochemical markers useful for the evaluation of tumors with chordoid morphology were D2-40, EMA, cytokeratin, and GFAP. D2-40 was a true chondroid marker to be useful for the differential diagnosis with chordoma
11. our cell transfection data do indicate that the p.Glu223Gln change has an effect on GFAP polymerization, albeit a modest one
12. Population variation in GFAP with varying Alzheimer lesion burdens suggests that they are not the only driver for astrogliosis
13. NFI family of transcription factors plays a key role in the regulation of both the B-FABP and GFAP genes in malignant glioma cells.
14. This study indicates that the mechanism of GFAP aggregation depends on the domain in which the point mutation(R239C and R416W) is located.
15. Data show tha GFAP levels are significantly higher in intracerebral hemorrhage patients than in ischemic stroke patients.
16. EAAT-1 is superior to GFAP as a helpful diagnostic tool for cancer in choroid plexus samples
17. This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia.
18. In Alexander disease mouse models that loss of Cryab results in increased mortality, whereas elevation of Cryab rescues animals from terminal seizures.
19. Downregulated by cytomegalovirus IE1 and IE2 proteins during HCMV infection in U373MG cell line.
20. There were strong correlations between the cerebrospinal fluid glial fibrillar acidic protein level and expanded disability status scales or spinal lesion length in neuromyelitis optica
2. GFAP+1 expressing astrocytes may have a specific role in Alzheimer's disease
3. Results identified glial fibrillary acidic protein that showed elevated levels of protein carbonyls in inferior parietal lobule (IPL) from subjects with early stage-Alzheimer's disease.
4. Determination of relative levels of MBP and GFAP mRNAs may be useful for glial tumors recognition.
5. GFAP-delta may be a useful diagnostic marker for the evaluation of functional cataplasia or proliferation of astrocytic tumor.
6. GFAP is a potential marker for tumors with cartilaginous differentiation.
7. Novel variant (S398F) in the glial fibrillary acidic protein gene is found in a patient with Alexander disease.
8. GFAP-delta is expressed in a variety of pathologies associated with epilepsy, increased expression is shown in regions of astrocytic gliosis
9. GFAP had higher mean expression levels across regions in all psychiatric groups relative to normal control.
10. the best immunohistochemical markers useful for the evaluation of tumors with chordoid morphology were D2-40, EMA, cytokeratin, and GFAP. D2-40 was a true chondroid marker to be useful for the differential diagnosis with chordoma
11. our cell transfection data do indicate that the p.Glu223Gln change has an effect on GFAP polymerization, albeit a modest one
12. Population variation in GFAP with varying Alzheimer lesion burdens suggests that they are not the only driver for astrogliosis
13. NFI family of transcription factors plays a key role in the regulation of both the B-FABP and GFAP genes in malignant glioma cells.
14. This study indicates that the mechanism of GFAP aggregation depends on the domain in which the point mutation(R239C and R416W) is located.
15. Data show tha GFAP levels are significantly higher in intracerebral hemorrhage patients than in ischemic stroke patients.
16. EAAT-1 is superior to GFAP as a helpful diagnostic tool for cancer in choroid plexus samples
17. This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia.
18. In Alexander disease mouse models that loss of Cryab results in increased mortality, whereas elevation of Cryab rescues animals from terminal seizures.
19. Downregulated by cytomegalovirus IE1 and IE2 proteins during HCMV infection in U373MG cell line.
20. There were strong correlations between the cerebrospinal fluid glial fibrillar acidic protein level and expanded disability status scales or spinal lesion length in neuromyelitis optica
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Product Notes
The GFAP gfap (Catalog #AAA570104) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Glial fibrillary acidic protein / GFAP reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Glial fibrillary acidic protein / GFAP can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB). 6F2 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the GFAP gfap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glial fibrillary acidic protein / GFAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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