Western Blot (WB)
(Figure 1: Western blot analysis using GKAP mAb against HEK293 (1) and GKAP(AA: 490-663)-hIgGFc transfected HEK293 (2) cell lysate. )
Mouse anti-Human GKAP Monoclonal Antibody | anti-GKAP antibody
GKAP Antibody
Gene Names
GKAP1; FKSG21; GKAP42
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Affinity-Chromatography
Synonyms
GKAP; Monoclonal Antibody; GKAP Antibody; cGMP dependent protein kinase anchoring protein 42kDa; cGMP dependent protein kinase anchoring protein of 42 kDa; cGMP-dependent protein kinase-anchoring protein of 42 kDa; FKSG21; G kinase anchoring protein 1; G kinase-anchoring protein 1; gkap1; GKAP1_HUMAN; GKAP42; Protein kinase anchoring protein GKAP42; anti-GKAP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
GKAP antibody detects endogenous levels of total GKAP
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
315
Applicable Applications for anti-GKAP antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
Immunogen
Purified recombinant fragment of human GKAP expressed in E. Coli
Subcellular Location
Golgi Apparatus.
Tissue Specificity
Expressed in brain.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using GKAP mAb against HEK293 (1) and GKAP(AA: 490-663)-hIgGFc transfected HEK293 (2) cell lysate. )
Western Blot (WB)
(Figure 1: Western blot analysis using GKAP mAb against HEK293 (1) and GKAP(AA: 490-663)-hIgGFc transfected HEK293 (2) cell lysate. )
Related Product Information for anti-GKAP antibody
Function: Part of the postsynaptic scaffold in neuronal cells.
Subunit Structure: Interacts with PRKG1.
Similarity: Belongs to the GKAP1 family.
Subunit Structure: Interacts with PRKG1.
Similarity: Belongs to the GKAP1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 109 kDa
Predicted: 43 kDa
Predicted: 43 kDa
NCBI Official Full Name
G kinase-anchoring protein 1 isoform b
NCBI Official Synonym Full Names
G kinase anchoring protein 1
NCBI Official Symbol
GKAP1
NCBI Official Synonym Symbols
FKSG21; GKAP42
NCBI Protein Information
G kinase-anchoring protein 1
UniProt Protein Name
G kinase-anchoring protein 1
UniProt Gene Name
GKAP1
UniProt Synonym Gene Names
GKAP42
NCBI Description
This gene encodes a protein that is highly similar to the mouse cGMP-dependent protein kinase anchoring protein 42kDa. The mouse protein has been found to localize with the Golgi and recruit cGMP-dependent protein kinase I alpha to the Golgi in mouse testes. It is thought to play a role in germ cell development. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
Uniprot Description
Regulates insulin-dependent IRS1 tyrosine phosphorylation in adipocytes by modulating the availability of IRS1 to IR tyrosine kinase. Its association with IRS1 is required for insulin-induced translocation of SLC2A4 to the cell membrane. Involved in TNF-induced impairment of insulin-dependent IRS1 tyrosine phosphorylation.
Research Articles on GKAP
Similar Products
Product Notes
The GKAP gkap1 (Catalog #AAA9602775) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GKAP Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GKAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000 ICC: 1:200-1:1000. Researchers should empirically determine the suitability of the GKAP gkap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GKAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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