Mouse anti-Human GILT Monoclonal Antibody | anti-GILT antibody
GILT, Human, mAb MaP.IP30
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
Synonyms
GILT; Monoclonal Antibody; Human; mAb MaP.IP30; clone MaP.IP30; Lfi30; Interferon-gamma-inducible lysosomal reductase; anti-GILT antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
MaP.IP30
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-GILT antibody
Flow Ctometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
FC: Antibody MaP.IP30 stains the intra and extracellular domain of GILT. For intracellular staining U937 cells were permeabilized with buffer containing 0.05% saponin. The cells were fixed in 3.7% formaldehyde before staining.
For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 2ug/250000 cells.
For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 2ug/250000 cells.
Immunogen
Partial purified IP30 (purified from human B-LCL)
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Related Product Information for anti-GILT antibody
Antibodies derived from clone MAP.IP30 recognize human gamma-interferon-inducible lysosomal thiol reductase (GILT). GILT facilitates major histocompatibility complex (MHC) class II-restricted processing through reduction of protein disulfide bonds in the endocytic pathway. GILT can also facilitate the transfer of disulfide-containing antigens into the cytosol, enhancing their cross-presentation by MHC class I. Human GILT consists of 261 amino acids with a 37 amino acid signal sequence and a 224 amino acid precursor form. In general, the 35 kDa precursor is tagged with mannose-6- phosphate (M6P) residues and transported to the endocytic pathway via the M6P receptor (M6PR). A small fraction of the precursor is secreted as a disulfide-linked dimer. In early endosomes, N- and C-terminal pro-peptides are cleaved to generate a 28 kDa mature form. The mature form of GILT is localized to late endosomes and lysosomes and has maximal reductase activity at the acidic pH found in these compartments. GILT is constitutively expressed in professional antigen presenting cells (APC). The constitutive expression of GILT in APCs is likely to account for enhanced reduction of proteins in the late endosomal, lysosomal, and phagosomal compartments. A combination of GILT-mediated reduction and proteolysis in the these compartments can enhance the generation of class II epitopes as well as facilitate the translocation of internalized antigens into the cytosol for proteosomal processing and cross-presentation. The protein is also constitutively expressed in thymocytes, mature T cells, and some fibroblasts. IFN-g plays is the key factor in inducing expression of MHC class II and other components of the class II-restricted processing pathway. IFN-g signals through a specific cell surface receptor followed by activation of JAK/STAT1 signalling.
References
1. Arunachalam, B et al; Intracellular formation and cell surface expression of a complex of an intact lysosomal protein and MHC class II molecules. J Immunol 1998, 160: 5797 2. Lackman, R et al; Exposure of the promonocytic cell line THP-1 to Escherichia coli induces IFN-gamma-inducible lysosomal thiol reductase expression by inflammatory cytokines. J Immunol 2006, 177: 4833.
3. Hastings, K et al; Functional requirements for the lysosomal thiol reductase GILT in MHC class II-restricted antigen processing. J Immunol 2006, 177: 8569
4. Lackman, R et al; Innate immune recognition triggers secretion of lysosomal enzymes by macrophages. Traffic 2007, 8: 1179
5. Taraszka Hastings, K et al ; Functional requirements for the lysosomal thiol reductase GILT in MHC Class II-Restricted Antigen Processing. Journal of immunology 2006, 177: 8569
6. Uyen, T et al; Role of the C-terminal propeptide in the activity and maturation of -interferon-inducible lysosomal thiolreductase (GILT). PNAS 2002, 99: 12298
3. Hastings, K et al; Functional requirements for the lysosomal thiol reductase GILT in MHC class II-restricted antigen processing. J Immunol 2006, 177: 8569
4. Lackman, R et al; Innate immune recognition triggers secretion of lysosomal enzymes by macrophages. Traffic 2007, 8: 1179
5. Taraszka Hastings, K et al ; Functional requirements for the lysosomal thiol reductase GILT in MHC Class II-Restricted Antigen Processing. Journal of immunology 2006, 177: 8569
6. Uyen, T et al; Role of the C-terminal propeptide in the activity and maturation of -interferon-inducible lysosomal thiolreductase (GILT). PNAS 2002, 99: 12298
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Product Notes
The GILT (Catalog #AAA584695) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GILT, Human, mAb MaP.IP30 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GILT can be used in a range of immunoassay formats including, but not limited to, Flow Ctometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP). FC: Antibody MaP.IP30 stains the intra and extracellular domain of GILT. For intracellular staining U937 cells were permeabilized with buffer containing 0.05% saponin. The cells were fixed in 3.7% formaldehyde before staining. For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 2ug/250000 cells. Researchers should empirically determine the suitability of the GILT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GILT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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