Western Blot (WB)
(Western Blot (WB) analysis of GFAP Mouse mAb at 1:5000 dilutionLane1:The brain tissue lysate of RatLane2:The brain tissue lysate of Human)
Mouse GFAP Monoclonal Antibody | anti-GFAP antibody
GFAP Mouse Monoclonal Antibody
IHC: 1:200
ICC: 1:200
FC/FACS: 1:200
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of GFAP Mouse mAb at 1:5000 dilutionLane1:The brain tissue lysate of RatLane2:The brain tissue lysate of Human)
Western Blot (WB)
(Western Blot (WB) analysis of GFAP Mouse mAb at 1:5000 dilutionLane1:The brain tissue lysate of RatLane2:The brain tissue lysate of Human)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GFAPantibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GFAPantibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining of GFAP in A172 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Immunocytochemistry (ICC)
(ICC staining of GFAP in A172 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Immunocytochemistry (ICC)
(ICC staining of GFAP in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Immunocytochemistry (ICC)
(ICC staining of GFAP in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with GFAP antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with GFAP antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Uniprot Description
GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: membrane; cytoplasm; intermediate filament; cytosol
Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding
Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development
Disease: Alexander Disease
Research Articles on GFAP
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Product Notes
The GFAP gfap (Catalog #AAA3014416) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GFAP Mouse Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GFAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:2000-1:5000 IHC: 1:200 ICC: 1:200 FC/FACS: 1:200. Researchers should empirically determine the suitability of the GFAP gfap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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