Testing Data
Mouse General Transcription Factor IIE, Polypeptide 2beta, 34kD Monoclonal Antibody | anti-GTF2E2 antibody
General Transcription Factor IIE, Polypeptide 2beta, 34kD (GTF2E2, TF2E2, TFIIE-B)
Gene Names
GTF2E2; FE; TF2E2; TFIIE-B
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
General Transcription Factor IIE; Polypeptide 2beta; 34kD; Monoclonal Antibody; 34kD (GTF2E2; TF2E2; TFIIE-B); Anti -General Transcription Factor IIE; anti-GTF2E2 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
8C160
Specificity
Recognizes human General Transcription Factor IIE, Polypeptide 2beta, 34kD.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Applicable Applications for anti-GTF2E2 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blotting.
Dilution: Western Blotting: 1:1000-1:2000
Dilution: Western Blotting: 1:1000-1:2000
Immunogen
Partial sequence of recombinant full-length protein to human General Transcription Factor IIE, Polypeptide 2beta, 34kD
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
Testing Data
Western Blot (WB)
(Detection of GTF2E2 by Western blot. Samples: Whole cell lysate from human HEK293 (H, 25 μg) , mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. Predicted molecular weight: 33 kD)
Western Blot (WB)
(Detection of GTF2E2 by Western blot. Samples: Whole cell lysate from human HEK293 (H, 25 μg) , mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. Predicted molecular weight: 33 kD)
Immunostaining (IS)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS600737 mAb.)
Immunostaining (IS)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with MBS600737 mAb.)
Product Categories/Family for anti-GTF2E2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
33,044 Da
NCBI Official Full Name
general transcription factor IIE, polypeptide 2, beta 34kDa, isoform CRA_c
NCBI Official Synonym Full Names
general transcription factor IIE, polypeptide 2, beta 34kDa
NCBI Official Symbol
GTF2E2
NCBI Official Synonym Symbols
FE; TF2E2; TFIIE-B
NCBI Protein Information
transcription initiation factor IIE subunit beta; TFIIE-beta; OTTHUMP00000225416; general transcription factor IIE subunit 2
UniProt Protein Name
Transcription initiation factor IIE subunit beta
Protein Family
UniProt Gene Name
GTF2E2
UniProt Synonym Gene Names
TF2E2
UniProt Entry Name
T2EB_HUMAN
Uniprot Description
GTF2E2: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Belongs to the TFIIE beta subunit family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 8p12
Cellular Component: nucleoplasm; cytoplasm; transcription factor TFIIE complex; nucleus
Molecular Function: protein binding; DNA binding
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; viral reproduction; RNA elongation from RNA polymerase II promoter; gene expression
Research Articles on GTF2E2
Similar Products
Product Notes
The GTF2E2 gtf2e2 (Catalog #AAA600737) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The General Transcription Factor IIE, Polypeptide 2beta, 34kD (GTF2E2, TF2E2, TFIIE-B) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's General Transcription Factor IIE, Polypeptide 2beta, 34kD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blotting. Dilution: Western Blotting: 1:1000-1:2000. Researchers should empirically determine the suitability of the GTF2E2 gtf2e2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "General Transcription Factor IIE, Polypeptide 2beta, 34kD, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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