Mouse Gemin 3 Monoclonal Antibody

Mouse Anti-Gemin 3 [12H12]

Cat. Num. AAA375230

• Reactivity: Human, Rat, Mouse, Hamster
• Applications: ELISA, Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Purity: Protein A
• Synonyms: Gemin 3; Monoclonal Antibody; Mouse Anti-Gemin 3 [12H12]; Component of gems 3 antibody; DDX 20 antibody; DDX20 antibody; DDX20_HUMAN antibody; DEAD (Asp Glu AlaAsp) box polypeptide 20 antibody; DEAD (Asp-Glu-Ala-Asp) box polypeptide 20 antibody; DEAD box protein 20antibody; DEAD box protein DP 103 antibody; DEAD box protein DP103 antibody; DEAD-box protein DP103antibody; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody; DEAD/H (Asp Glu Ala Asp/His) boxpolypeptide 20 antibody; DEAD/H BOX 20 antibody; DKFZP434H052 antibody; DP 103 antibody; DP103 antibody; Gemin-3antibody; Gemin3 antibody; Probable ATP dependent RNA helicase DDX20 antibody; Probable ATP-dependent RNA helicaseDDX20 antibody; SMN interacting protein antibody; anti-Gemin 3 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse, Hamster
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 12H12
• Purity/Purification: Protein A
• Form/Format: Purified antibody (from supernatant) containing PBS 0.1% sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-Gemin 3 antibody: ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try: WB: Use at an assay dependent dilution. Detects a band of approximately 105 kDa (predicted molecular weight: 99 kDa). Flow Cyt: Use 1ug for 106 cells. ICC/IF: 1/50.
• Target Antigen: Gemin 3
• Immunogen: Recombinant 6His-tag COOH-terminal domain of Gemin3 (amino acids 368-548)
• Myeloma/fusion Partners: SP2/0
• Research Areas: Neuroscience; signal transduction; Splicing

Immunohistochemistry (IHC)

References

Mahmoudi S et al. WRAP53 is essential for Cajal body formation and for targeting the survival of motor neuron complex to Cajal bodies. PLoS Biol 8:e1000521 (2010). WB ; HumanPUBMED Bradrick SS & Gromeier M Identification of gemin5 as a novel 7-methylguanosine cap-binding protein. PLoS One 4:e7030 (2009). WB ; HumanPUBMED Castell³ A et al. RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage. J Cell Sci 122:3799-809 (2009). WB ; HumanPUBMED Walker MP et al. SMN complex localizes to the sarcomeric Z-disc and is a proteolytic target of calpain. Hum Mol Genet 17:3399-410 (2008). ICC/IF ; HamsterPUBMED Morency E et al. A novel cell response triggered by interphase centromere structural instability. J Cell Biol 177:757-68 (2007). ICC/IF ; HumanPUBMED Murashov AK et al. RNAi pathway is functional in peripheral nerve axons. FASEB J. 2007 Mar;21(3):656-70. Epub 2007 Jan 5.ICC/IF, IHC, WB; Rat MousePMID: 17209129 Hamamoto S et al. Identification of a novel human immunodeficiency virus type 1 integrase interactor, Gemin2, that facilitates efficient viral cDNA synthesis in vivo. J Virol. 2006 Jun;80(12):5670-7.WB; HumanPMID: 16731905 Zhang H et al. Multiprotein complexes of the survival of motor neuron protein SMN with Gemins traffic to neuronal processes and growth cones of motor neurons. J Neurosci 26:8622-32 (2006)PUBMED Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems. Charroux et.al (J Cell Biol,Dec 1999)PUBMED