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Immunohistochemistry (IHC) (IHC with ACBD3 antibody on frozen human tonsil.)

Mouse GCP60 / ACBD3 Monoclonal Antibody | anti-GCP60 antibody

Mouse Monoclonal [clone 2G2] (IgG1,k) to Human GCP60 / ACBD3

Gene Names
ACBD3; PAP7; GCP60; GOCAP1; GOLPH1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
GCP60 / ACBD3; Monoclonal Antibody; Mouse Monoclonal [clone 2G2] (IgG1; k) to Human GCP60 / ACBD3; Anti-GCP60 / ACBD3 Antibody (clone 2G2) IHC-plus; ACBD3; Golgi phosphoprotein 1; GCP60; GOCAP1; Golgi resident protein GCP60; GOLPH1; PAP7; Human GCP60; anti-GCP60 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2G2
Purity/Purification
Protein A Purified
Form/Format
PBS, pH 7.4.
Concentration
0.45 mg/ml (varies by lot)
Sequence Length
528
Applicable Applications for anti-GCP60 antibody
Immunohistochemistry (IHC - Frozen), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IF (10 ug/ml), IHC-Fr (1:50), WB
Usage: Western Blot (Cell lysate) - positive control HeLa. Western Blot (Cell lysate) - positive control PC-12. Western Blot (Cell lysate) - positive control Raw 264. Western Blot (Cell lysate) - positive control NIH/3T3. Western Blot (Transfected lysate) -...
Usage Not Recommended
Immunohistchemistry (IHC-P)
Target Species
Human
Immunogen Description
ACBD3 (NP_073572, 73 a.a. ~ 172 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
GCP60 / ACBD3 antibody was raised against aCBD3 (NP_073572, 73 a.a. ~ 172 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(IHC with ACBD3 antibody on frozen human tonsil.)

Immunohistochemistry (IHC) (IHC with ACBD3 antibody on frozen human tonsil.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ACBD3 on HeLa cell (antibody concentration 10 ug/ml).)

Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to ACBD3 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(ACBD3 monoclonal antibody, clone 2G2. Western blot of ACBD3 expression in Raw 264.7.)

Western Blot (WB) (ACBD3 monoclonal antibody, clone 2G2. Western blot of ACBD3 expression in Raw 264.7.)

Western Blot (WB)

(ACBD3 monoclonal antibody, clone 2G2. Western blot of ACBD3 expression in NIH/3T3.)

Western Blot (WB) (ACBD3 monoclonal antibody, clone 2G2. Western blot of ACBD3 expression in NIH/3T3.)

Western Blot (WB)

(ACBD3 monoclonal antibody, clone 2G2 Western blot of ACBD3 expression in HeLa.)

Western Blot (WB) (ACBD3 monoclonal antibody, clone 2G2 Western blot of ACBD3 expression in HeLa.)

ELISA (EIA)

(Detection limit for recombinant GST tagged ACBD3 is approximately 0.1 ng/ml as a capture antibody.)

ELISA (EIA) (Detection limit for recombinant GST tagged ACBD3 is approximately 0.1 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60,593 Da
NCBI Official Full Name
Golgi resident protein GCP60
NCBI Official Synonym Full Names
acyl-CoA binding domain containing 3
NCBI Official Symbol
ACBD3
NCBI Official Synonym Symbols
PAP7; GCP60; GOCAP1; GOLPH1
NCBI Protein Information
Golgi resident protein GCP60; golgi phosphoprotein 1; PKA (RIalpha)-associated protein; PBR- and PKA-associated protein 7; golgi complex associated protein 1, 60kDa; acyl-Coenzyme A binding domain containing 3; peripheral benzodiazepine receptor-associated protein PAP7
UniProt Protein Name
Golgi resident protein GCP60
UniProt Gene Name
ACBD3
UniProt Synonym Gene Names
GCP60; GOCAP1; GOLPH1; GOCAP1; GOLPH1
UniProt Entry Name
GCP60_HUMAN

NCBI Description

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in the maintenance of Golgi structure and function through its interaction with the integral membrane protein giantin. It may also be involved in the hormonal regulation of steroid formation. [provided by RefSeq, Jul 2008]

Uniprot Description

ACBD3: Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells.

Chromosomal Location of Human Ortholog: 1q42.12

Cellular Component: Golgi membrane; Golgi apparatus; mitochondrion; membrane; integral to membrane

Molecular Function: protein binding; acyl-CoA binding

Biological Process: transport; steroid biosynthetic process

Research Articles on GCP60

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Product Notes

The GCP60 acbd3 (Catalog #AAA249181) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 2G2] (IgG1,k) to Human GCP60 / ACBD3 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GCP60 / ACBD3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Frozen), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). ELISA, IF (10 ug/ml), IHC-Fr (1:50), WB Usage: Western Blot (Cell lysate) - positive control HeLa. Western Blot (Cell lysate) - positive control PC-12. Western Blot (Cell lysate) - positive control Raw 264. Western Blot (Cell lysate) - positive control NIH/3T3. Western Blot (Transfected lysate) -.. Researchers should empirically determine the suitability of the GCP60 acbd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCP60 / ACBD3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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