Mouse anti-Human GCLM Monoclonal Antibody | anti-GCLM antibody

GCLM (Glutamate-cysteine Ligase Modifier Subunit, Gamma-glutamylcysteine Synthetase Regulatory Subunit, Gamma-ECS Regulatory Subunit, GCS Light Chain, Glutamate-cysteine Ligase Regulatory Subunit, GLCLR) (AP)

Cat. Num. AAA6131441

• Gene Names: GCLM; GLCLR
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GCLM; Monoclonal Antibody; GCLM (Glutamate-cysteine Ligase Modifier Subunit; Gamma-glutamylcysteine Synthetase Regulatory Subunit; Gamma-ECS Regulatory Subunit; GCS Light Chain; Glutamate-cysteine Ligase Regulatory Subunit; GLCLR) (AP); anti-GCLM antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2B8
• Specificity: Recognizes human GCLM.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-GCLM antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-274 from human GCLM (AAH41809) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSGASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (55.88kD).)

Western Blot (WB)

(GCLM monoclonal antibody, Western Blot analysis of GCLM expression in HeLa.)

Testing Data

(Detection limit for recombinant GST tagged GCLM is ~1ng/ml as a capture antibody.)

Related Product Information for anti-GCLM antibody

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Product Categories/Family for anti-GCLM antibody

Antibodies; Enzymes; Synthase

References

1. Protection against 2-chloroethyl ethyl sulfide (CEES) - Induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway. Abel EL, Bubel JD, Simper MS, Powell L, McClellan SA, Andreeff M, Macleod MC, Digiovanni J.Toxicol Appl Pharmacol. 2011 Jun 23. 2. Molecular and Functional Characterization of Gastrula Organizer Cells Derived from Human Embryonic Stem Cells. Sharon N, Mor I, Golan-Lev T, Fainsod A, Benvenisty N.Stem Cells. 2011 Feb 15. doi: 10.1002/stem.621. 3. Diversity in Antioxidant Response Enzymes in Progressive Stages of Human Nonalcoholic Fatty Liver Disease. Hardwick RN, Fisher CD, Canet MJ, Lake AD, Cherrington NJ.Drug Metab Dispos. 2010 Dec;38(12):2293-301. Epub 2010 Aug 30. 4. Induction of Antioxidant Enzymes by Curcumin and Its Analogues in Human Islets: Implications in Transplantation. Balamurugan AN, Akhov L, Selvaraj G, Pugazhenthi S.Pancreas. 2009 May;38(4):454-60.

NCBI and Uniprot Product Information

• NCBI GI #: 27469698
• NCBI GeneID: 2730
• Molecular Weight: 28,135 Da
• NCBI Official Full Name: Homo sapiens glutamate-cysteine ligase, modifier subunit, mRNA
• NCBI Official Synonym Full Names: glutamate-cysteine ligase modifier subunit
• NCBI Official Symbol: GCLM
• NCBI Official Synonym Symbols: GLCLR
• NCBI Protein Information: glutamate--cysteine ligase regulatory subunit
• Protein Family: Glutamate--cysteine ligase regulatory

NCBI Description

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]