Western Blot (WB)
(GBF1 monoclonal antibody (M04), clone 4B2. Western Blot analysis of GBF1 expression in HeLa.)
Mouse GBF1 Monoclonal Antibody | anti-GBF1 antibody
GBF1 (Golgi-Specific brefeldin A resistant Guanine Nucleotide Exchange Factor 1, ARF1GEF, FLJ21263, FLJ21500, KIAA0248, MGC134877, MGC134878) (FITC)
Gene Names
GBF1; ARF1GEF
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Purified
Synonyms
GBF1; Monoclonal Antibody; GBF1 (Golgi-Specific brefeldin A resistant Guanine Nucleotide Exchange Factor 1; ARF1GEF; FLJ21263; FLJ21500; KIAA0248; MGC134877; MGC134878) (FITC); Golgi-Specific brefeldin A resistant Guanine Nucleotide Exchange Factor 1; MGC134878; anti-GBF1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4B2
Specificity
Recognizes GBF1.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Sequence Length
1859
Applicable Applications for anti-GBF1 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
GBF1 (NP_004184.1, 101aa-209aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
THEGTAEGMENMADAVTHARFVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKSAEHTLVDMVQLLFTRLPQFKEEPKNYVGT
Conjugate
FITC
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(GBF1 monoclonal antibody (M04), clone 4B2. Western Blot analysis of GBF1 expression in HeLa.)
Western Blot (WB)
(GBF1 monoclonal antibody (M04), clone 4B2. Western Blot analysis of GBF1 expression in HeLa.)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to GBF1 on HeLa cell. [antibody concentration 10 ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to GBF1 on HeLa cell. [antibody concentration 10 ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to GBF1 on HeLa cell. [antibody concentration 10 ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to GBF1 on HeLa cell. [antibody concentration 10 ug/ml])
Related Product Information for anti-GBF1 antibody
Mouse monoclonal antibody raised against a partial recombinant GBF1.
Product Categories/Family for anti-GBF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform 1
NCBI Official Synonym Full Names
golgi brefeldin A resistant guanine nucleotide exchange factor 1
NCBI Official Symbol
GBF1
NCBI Official Synonym Symbols
ARF1GEF
NCBI Protein Information
Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
UniProt Protein Name
Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
Protein Family
UniProt Gene Name
GBF1
UniProt Synonym Gene Names
KIAA0248; BFA-resistant GEF 1
UniProt Entry Name
GBF1_HUMAN
NCBI Description
This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010]
Research Articles on GBF1
Similar Products
Product Notes
The GBF1 gbf1 (Catalog #AAA6178650) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GBF1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GBF1 gbf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GBF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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