Mouse GABPA Monoclonal Antibody | anti-GABPA antibody

GABPA (GA Binding Protein Transcription Factor, alpha Subunit 60kD, E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A) (AP)

Cat. Num. AAA6163577

• Gene Names: GABPA; NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: GABPA; Monoclonal Antibody; GABPA (GA Binding Protein Transcription Factor; alpha Subunit 60kD; E4TF1-60; E4TF1A; NFT2; NRF2; NRF2A) (AP); GA Binding Protein Transcription Factor; NRF2A; anti-GABPA antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 5C8
• Specificity: Recognizes GABPA.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-GABPA antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: GABPA (NP_002031, 1aa-100aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MTKREAEELIEIEIDGTEKAECTEESIVEQTYAPAECVSQAIDINEPIGNLKKLLEPRLQCSLDAHEICLQDIQLDPERSLFDQGVKTDGTVQLSVQVIS
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GABPA monoclonal antibody (M07), clone 5C8 Western Blot analysis of GABPA expression in Hela S3 NE.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])

Testing Data

(Detection limit for recombinant GST tagged GABPA is approximately 3ng/ml as a capture antibody.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to GABPA on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to GABPA on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])

Related Product Information for anti-GABPA antibody

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. [provided by RefSeq]

Product Categories/Family for anti-GABPA antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 34452721
• NCBI GeneID: 2551
• NCBI Accession #: NP_002031
• NCBI GenBank Nucleotide #: NM_002040
• UniProt Accession #: Q06546
• Molecular Weight: 51kDa
• NCBI Official Full Name: GA-binding protein alpha chain
• NCBI Official Synonym Full Names: GA binding protein transcription factor subunit alpha
• NCBI Official Symbol: GABPA
• NCBI Official Synonym Symbols: NFT2; NRF2; NRF2A; E4TF1A; E4TF1-60; RCH04A07
• NCBI Protein Information: GA-binding protein alpha chain
• UniProt Protein Name: GA-binding protein alpha chain
• Protein Family: GA-binding protein
• UniProt Gene Name: GABPA
• UniProt Synonym Gene Names: E4TF1A; GABP subunit alpha
• UniProt Entry Name: GABPA_HUMAN

NCBI Description

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

GABPA: Transcription factor capable of interacting with purine rich repeats (GA repeats). Necessary for the expression of the Adenovirus E4 gene. Belongs to the ETS family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 21q21.3

Cellular Component: nucleoplasm; nuclear chromatin; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; transcription coactivator activity; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; mitochondrion organization and biogenesis; in utero embryonic development; organelle organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; negative regulation of megakaryocyte differentiation