Western Blot (WB)
(Western Blot analysis of Rat brain membrane lysate showing detection of GABA B Receptor 1 protein using Mouse Anti-GABA B Receptor 1 Monoclonal Antibody, Clone S93A-49. Primary Antibody: Mouse Anti-GABA B Receptor 1 Monoclonal Antibody at 1:1000.)
Mouse GABA(B) Receptor 1 Monoclonal Antibody | anti-Gabbr1 antibody
GABA-B Receptor 1 Antibody: RPE
Western Blot (WB)
(Western Blot analysis of Rat brain membrane lysate showing detection of GABA B Receptor 1 protein using Mouse Anti-GABA B Receptor 1 Monoclonal Antibody, Clone S93A-49. Primary Antibody: Mouse Anti-GABA B Receptor 1 Monoclonal Antibody at 1:1000.)
Western Blot (WB)
(Western Blot analysis of Rat brain membrane lysate showing detection of GABA B Receptor 1 protein using Mouse Anti-GABA B Receptor 1 Monoclonal Antibody, Clone S93A-49. Primary Antibody: Mouse Anti-GABA B Receptor 1 Monoclonal Antibody at 1:1000.)
Scientific Background: GABA (gamma-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).
NCBI and Uniprot Product Information
NCBI Description
metabotropic G-protein-coupled receptor for gamma-aminobutyric acid (GABA) [RGD, Feb 2006]
Uniprot Description
GABBR1: Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high- voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Activated by (-)-baclofen, cgp27492 and blocked by phaclofen. Heterodimer of GABA-B-R1 and GABA-B-R2. Neither of which is effective on its own and homodimeric assembly does not seem to happen. Isoform 1E (without C-terminal intracellular domain) is unable to dimerize via a coiled-coil interaction with GABA-B-R2. Interacts with the leucine zipper of the C-terminal bZIP domain of ATF4 via its C-terminal region. Interacts with JAKMIP1. Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland. Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: GPCR, family 3; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral
Cellular Component: extracellular space; endoplasmic reticulum membrane; neuron projection; intracellular membrane-bound organelle; integral to plasma membrane; integral to membrane; dendritic spine; axolemma; lipid raft; presynaptic membrane; postsynaptic membrane; synaptic vesicle; cell soma; membrane; cytoplasm; mitochondrial membrane; dendritic shaft; cell junction
Molecular Function: protein binding; GABA-B receptor activity; transcription factor binding
Biological Process: response to nicotine; regulation of glutamate secretion; negative regulation of adenylate cyclase activity; negative regulation of gamma-aminobutyric acid secretion; positive regulation of glutamate secretion; negative regulation of synaptic transmission; osteoblast differentiation; positive regulation of growth hormone secretion; negative regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; response to ethanol; negative regulation of epinephrine secretion; G-protein signaling, adenylate cyclase inhibiting pathway; negative regulation of dopamine secretion; regulation of cAMP biosynthetic process; gamma-aminobutyric acid signaling pathway
Research Articles on Gabbr1
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Product Notes
The Gabbr1 gabbr1 (Catalog #AAA800614) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GABA-B Receptor 1 Antibody: RPE reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GABA(B) Receptor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). 1:1000 (WB). Researchers should empirically determine the suitability of the Gabbr1 gabbr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GABA(B) Receptor 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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