Mouse anti-Human FSH-beta (Follicle Stimulating Hormone-beta) Monoclonal Antibody | anti-FSHB antibody

FSH-beta (Follicle Stimulating Hormone-beta) Mouse Monoclonal Antibody

Cat. Num. AAA439339

• Gene Names: FSHB; HH24
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: FSH-beta (Follicle Stimulating Hormone-beta); Monoclonal Antibody; FSH-beta (Follicle Stimulating Hormone-beta) Mouse Monoclonal Antibody; Follicle stimulating hormone beta polypeptide; Follicle stimulating hormone beta subunit; Follitropin beta chain; Follitropin subunit beta; FSH-B; FSH-beta; FSHB; FSHbeta; anti-FSHB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM107
• Specificity: This MAb reacts with a protein of 22kDa, identified as beta sub-unit of FSH. It does not cross react with the alpha sub-unit. Follicle stimulating hormone (FSH) is a hormone synthesized and secreted by gonadotrophs in the anterior pituitary gland. In the ovary, FSH stimulates the growth of immature Graafian follicles to maturation. As the follicle grows, it releases inhibin, which deactivates the FSH production. In men, FSH enhances the production of androgen-binding protein by the Sertoli cells of the testis and is critical for spermatogenesis. FSH and LH act synergistically in reproduction. FSH is a useful marker in the classification of pituitary tumors and the study of pituitary disease.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 129

• Applicable Applications for anti-FSHB antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml; 30 min RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic, Secreted
• Immunogen: Full length purified Human FSH
• Hu-Chromosome Location: 11p13
• Positive Control: Anterior Pituitary
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Pituitary stained with FSH-beta Monoclonal Antibody (SPM107).)

References

Schmidt M et al. Pathol Res Pract. 2001;197(10):663-9 La Rosa S et al. Virchows Arch. 2000 Sep;437(3):264-9 Zheng W et al. Gynecol Oncol. 2000 jan;76(1):80-8

NCBI and Uniprot Product Information

• NCBI GI #: 4503791
• NCBI GeneID: 2488
• NCBI Accession #: NP_000501.1
• NCBI GenBank Nucleotide #: NM_000510.2
• UniProt Accession #: P01225; Q14D61; A2TF08; A5JVV3
• Molecular Weight: 22kDa
• NCBI Official Full Name: follitropin subunit beta
• NCBI Official Synonym Full Names: follicle stimulating hormone beta subunit
• NCBI Official Symbol: FSHB
• NCBI Official Synonym Symbols: HH24
• NCBI Protein Information: follitropin subunit beta
• UniProt Protein Name: Follitropin subunit beta
• Protein Family: Follitropin
• UniProt Gene Name: FSHB
• UniProt Synonym Gene Names: FSH-B; FSH-beta
• UniProt Entry Name: FSHB_HUMAN

NCBI Description

The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

FSHB: Stimulates development of follicle and spermatogenesis in the reproductive organs. Defects in FSHB are a cause of isolated follicle- stimulating hormone deficiency (IFSHD). Selective follicle-stimulating hormone deficiency is an uncommon cause of infertility, producing amenorrhea and hypogonadism in women and oligo or azoospermia with normal testosterone levels in normally virilised men. Belongs to the glycoprotein hormones subunit beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: cytoplasm; extracellular region

Molecular Function: follicle-stimulating hormone activity; hormone activity; protein binding

Biological Process: female gamete generation; female pregnancy; peptide hormone processing; progesterone biosynthetic process; signal transduction; transforming growth factor beta receptor signaling pathway

Disease: Follicle-stimulating Hormone Deficiency, Isolated