Mouse FOXP3 Monoclonal Antibody | anti-FOXP3 antibody

FOXP3

Cat. Num. AAA438434

• Gene Names: FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER
• Reactivity: Human, Monkey, Mouse.
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
• Synonyms: FOXP3; Monoclonal Antibody; JM2; AIID; DIETER; IPEX; MGC141961; MGC141963; PIDX; XPID; FOXP3delta7; forkhead box protein P3; immune dysregulation; polyendocrinopathy; enteropathy; X-linked; immunodeficiency; scurfin; anti-FOXP3 antibody

• Host: Mouse
• Reactivity: Human, Monkey, Mouse.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 3G3
• Specificity: Recognizes a protein of 47-55kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion (amino acids 2-136) of the protein. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 396

• Applicable Applications for anti-FOXP3 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology (IH)
• Application Notes: ELISA (For coating, order Ab without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunoprecipitation (0.5-1ug/500ug protein lysate); Immunohistology (Frozen) (0.5-1ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined.
• Localization: Predominantly nuclear, some cytoplasmic
• Positive Control: Tonsil, lymph node or Breast carcinoma
• Human Chromosomal Location: Xp11.23
• Immunogen: Full-length His-tagged mouse FOXP3 protein
• Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide
• Preparation and Storage: Store at 2 to 8 degree C. Antibody is stable for 24 months.

References

1. Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY: Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A. 2006;103(17):6659-64.
2. Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC: The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells. Cytometry A. 2009;75(12):1040-50.

NCBI and Uniprot Product Information

• NCBI GI #: 167466190
• NCBI GeneID: 50943
• NCBI Accession #: NP_001107849.1
• NCBI GenBank Nucleotide #: NM_001114377.1
• UniProt Accession #: Q9BZS1; O60827; Q14DD8; Q4ZH51; A5HJT1; B7ZLG0; B9UN80
• Molecular Weight: 47-55kDa
• NCBI Official Full Name: forkhead box protein P3 isoform b
• NCBI Official Synonym Full Names: forkhead box P3
• NCBI Official Symbol: FOXP3
• NCBI Official Synonym Symbols: JM2; AIID; IPEX; PIDX; XPID; DIETER
• NCBI Protein Information: forkhead box protein P3; scurfin; FOXP3delta7; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked
• UniProt Protein Name: Forkhead box protein P3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXP3
• UniProt Synonym Gene Names: IPEX
• UniProt Entry Name: FOXP3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Cell cycle regulation; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: protein complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; NF-kappaB binding; protein binding; protein homodimerization activity; NFAT protein binding; sequence-specific DNA binding; metal ion binding; histone deacetylase binding; transcription corepressor activity; transcription factor activity

Biological Process: negative regulation of interleukin-10 production; positive regulation of transcription, DNA-dependent; myeloid cell homeostasis; negative regulation of cytokine secretion; T cell receptor signaling pathway; negative regulation of interleukin-2 production; negative regulation of interleukin-6 production; T cell homeostasis; tolerance induction to self antigen; response to virus; negative regulation of transcription factor activity; negative regulation of immune response; cytokine production; negative regulation of T cell cytokine production; positive regulation of peripheral T cell tolerance induction; inhibition of NF-kappaB transcription factor; regulation of T cell anergy; negative regulation of interleukin-5 production; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; inhibition of CREB transcription factor; negative regulation of cytokine biosynthetic process; transcription from RNA polymerase II promoter; negative regulation of interleukin-4 production; T cell activation; negative regulation of activated T cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of interferon-gamma biosynthetic process; negative regulation of histone acetylation; positive regulation of interleukin-4 production; negative regulation of cell proliferation; CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell proliferation; B cell homeostasis; regulation of transcription, DNA-dependent; negative regulation of interferon-gamma production; positive regulation of T cell anergy; regulation of isotype switching to IgG isotypes; positive regulation of transforming growth factor-beta1 production; negative regulation of chronic inflammatory response; negative regulation of histone deacetylation; T cell mediated immunity; positive regulation of immature T cell proliferation in the thymus; negative regulation of tumor necrosis factor production; chromatin remodeling; negative regulation of interleukin-17 production; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; negative regulation of interleukin-2 biosynthetic process; positive regulation of histone acetylation

Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked