Mouse anti-Human FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) Monoclonal Antibody | anti-FRAP antibody
Monoclonal Antibody to FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP)
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]
Uniprot Description
mTOR: an atypical kinase belonging to the PIKK family of kinases. Is the catalytic subunit of two protein complexes, mTORC1 and mTORC2. mTORC1 activates S6K and inactivates 4E-BP1, up-regulating protein synthesis. mTORC1 contains Raptor, a positive regulatory subunit and scaffold for recruiting substrates, two negative regulators, PRAS40 and DEPTOR, and mLST8; it is a target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. mTORC2, a downstream effector of PI3K, is insensitive to rapamycin and activates Akt by phosphorylating a key activation site. mTORC2 contains regulatory subunits Rictor and mSIN1, PROTOR, mLST8, and the negative regulator DEPTOR. mTORC1 suppresses PI3K activity via a strong negative feedback loop that involves S6K1. Inhibiting mTORC1 ablates this negative feedback loop and potentiates PI3K signaling. Known inhibitors of mTOR include rapamycin, temsirolimus (CCI-779).
Protein type: ATYPICAL group; Autophagy; EC 2.7.11.1; FRAP subfamily; Kinase, protein; Motility/polarity/chemotaxis; PIKK family; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical
Chromosomal Location of Human Ortholog: 1p36.22
Cellular Component: cell soma; cytoplasm; cytosol; dendrite; endomembrane system; endoplasmic reticulum membrane; Golgi membrane; lysosomal membrane; lysosome; membrane; mitochondrial outer membrane; nucleoplasm; nucleus; phosphoinositide 3-kinase complex; PML body; TORC2 complex
Molecular Function: ATP binding; identical protein binding; kinase activity; phosphoprotein binding; protein binding; protein domain specific binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; ribosome binding
Biological Process: activation of protein kinase B activity; anoikis; brain development; cardiac muscle cell development; cardiac muscle contraction; cell aging; cell cycle arrest; cellular response to amino acid starvation; cellular response to hypoxia; cellular response to leucine starvation; cellular response to nutrient levels; cellular response to starvation; de novo'pyrimidine base biosynthetic process; DNA repair; energy reserve metabolic process; germ cell development; growth; heart morphogenesis; heart valve morphogenesis; long-term memory; maternal process involved in female pregnancy; mRNA stabilization; multicellular organism growth; negative regulation of autophagy; negative regulation of cell size; negative regulation of cholangiocyte apoptotic process; negative regulation of iodide transmembrane transport; negative regulation of macroautophagy; negative regulation of muscle atrophy; negative regulation of NFAT protein import into nucleus; negative regulation of protein phosphorylation; negative regulation of protein ubiquitination; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of actin filament polymerization; positive regulation of cholangiocyte proliferation; positive regulation of eating behavior; positive regulation of endothelial cell proliferation; positive regulation of epithelial to mesenchymal transition; positive regulation of gene expression; positive regulation of granulosa cell proliferation; positive regulation of keratinocyte migration; positive regulation of lamellipodium assembly; positive regulation of lipid biosynthetic process; positive regulation of myotube differentiation; positive regulation of neuron death; positive regulation of neuron maturation; positive regulation of nitric oxide biosynthetic process; positive regulation of oligodendrocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase B signaling; positive regulation of protein phosphorylation; positive regulation of sensory perception of pain; positive regulation of skeletal muscle hypertrophy; positive regulation of smooth muscle cell proliferation; positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase III promoter; positive regulation of translation; post-embryonic development; protein amino acid phosphorylation; protein autophosphorylation; protein catabolic process; regulation of actin cytoskeleton organization; regulation of brown fat cell differentiation; regulation of carbohydrate utilization; regulation of cell growth; regulation of cell size; regulation of cellular response to heat; regulation of fatty acid beta-oxidation; regulation of glycogen biosynthetic process; regulation of GTPase activity; regulation of macroautophagy; regulation of membrane permeability; regulation of myelination; regulation of osteoclast differentiation; regulation of response to food; response to amino acid; response to cocaine; response to insulin stimulus; response to morphine; response to nutrient; ruffle organization; signal transduction; social behavior; spinal cord development; TOR signaling; TORC1 signaling; visual learning; voluntary musculoskeletal movement; wound healing
Disease: Focal Cortical Dysplasia Of Taylor; Smith-kingsmore Syndrome
Research Articles on FRAP
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Product Notes
The FRAP mtor (Catalog #AAA2090651) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-5ug/mL Immunohistochemistry: 5-30ug/mL Immunocytochemistry: 5-30ug/mL. Researchers should empirically determine the suitability of the FRAP mtor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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