Rat anti-Rat Fibroblasts Monoclonal Antibody
Fibroblasts, Mouse, mAb ER-TR7
Reactivity
Rat
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Synonyms
Fibroblasts; Monoclonal Antibody; Mouse; mAb ER-TR7; anti-Fibroblasts antibody
Host
Rat
Reactivity
Rat
Clonality
Monoclonal
Isotype
Rat IgG2a
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-Fibroblasts antibody
F, Flow Cytometry (FC/FACS), Immunofluorescence (IF), P
Application Notes
Application Notes: F: Sections were stained using an indirect immunoperoxidase method (Ref.1).
Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:20.
Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:20.
Immunogen
Mouse thymic stromal cells
Positive Control
Spleen
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-Fibroblasts antibody
Product Description: Monoclonal antibody ER-TR7 recognizes with an intracellular component of mouse fibroblasts. Fibroblasts are the least specialized cells in the connective-tissue family. They are dispersed in connective tissue throughout the body, where they secrete a nonrigid extracellular matrix (ECM) that is rich in type I and/or type III collagen. Conective tissue consists of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts. The activation of fibroblasts by inflammatory stimuli results in their migration, proliferation and deposition of extracellular matrix components, important features involved in both wound healing and fibrosis.
The ER-TR7 antigen is a ubiquitous component of stromal (interstitial) matrix cartilage and of at least some basement membrane zones. The antigen detected is not a basement membrane component, nor any major collagen type or fibronectin. The antigen detected has a wider tissue distribution than reticulin. ER-TR7 detects an intracellular component of fibroblasts. Since ER-TR7 does not react with purified laminin, collagen types I-V, fibronectin, heparin sulfate proteoglycan, entactin or nidogen, it detects a hitherto uncharacterized antigen.
The monoclonal antibody ER-TR7 can be used to study the micro-anatomy of various organs. ER-TR7 outlines the various compartments of peripheral lymphoid organs by characteristic labeling patterns (no such compartments are found in central lymphoid organs). Furthermore ER-TR7 delineates various types of connective tissue compartments in nonlymphoid organs.
The antibody ER-TR7 detects reticular fibroblasts, which constitute the cellular framework of lymphoid and nonlymphoid organs and their products. ER-TR7 is useful to clearly delineated the follicles, periarteriolar lymphoid sheath and marginal zone; the major white pulp compartments. Furthermore in lymph nodes, the capsule, sinuses, follicles, paracortex and medullary cords are clearly delineated.
The ER-TR7 antigen is a ubiquitous component of stromal (interstitial) matrix cartilage and of at least some basement membrane zones. The antigen detected is not a basement membrane component, nor any major collagen type or fibronectin. The antigen detected has a wider tissue distribution than reticulin. ER-TR7 detects an intracellular component of fibroblasts. Since ER-TR7 does not react with purified laminin, collagen types I-V, fibronectin, heparin sulfate proteoglycan, entactin or nidogen, it detects a hitherto uncharacterized antigen.
The monoclonal antibody ER-TR7 can be used to study the micro-anatomy of various organs. ER-TR7 outlines the various compartments of peripheral lymphoid organs by characteristic labeling patterns (no such compartments are found in central lymphoid organs). Furthermore ER-TR7 delineates various types of connective tissue compartments in nonlymphoid organs.
The antibody ER-TR7 detects reticular fibroblasts, which constitute the cellular framework of lymphoid and nonlymphoid organs and their products. ER-TR7 is useful to clearly delineated the follicles, periarteriolar lymphoid sheath and marginal zone; the major white pulp compartments. Furthermore in lymph nodes, the capsule, sinuses, follicles, paracortex and medullary cords are clearly delineated.
References
1. Vliet van, E et al; Monoclonal antibodies to stromal cell types of the mouse thymus. Eur J Immunol 1984, 14: 524 2. Vliet van, E et al; Reticular fibroblasts in peripheral lymphoid organs identified by a monoclonal antibody. J Histochem Cytochem 1986, 34: 883
3. Kalled, S et al; Anti-CD40 ligand antibody treatment of SNF 1 mice with established nephritits: preservation of kidney function. J Immunol 1998, 120: 2158
4. Nolte, M et al; A conduit system distributes chemokines and small blood-borne molecules through the splenic white pulp. J Exp Med 2003, 3: 505
5. Svensson, M et al; Stromal cells direct local differentiation of regulatory dendritic cells. Immunity 2004, 21: 505
6. Alba, R et al; Biodistribution and retargeting of FX-binding ablated adenovirus serotype 5 vectors. Blood 2010 (Epub july 7).
3. Kalled, S et al; Anti-CD40 ligand antibody treatment of SNF 1 mice with established nephritits: preservation of kidney function. J Immunol 1998, 120: 2158
4. Nolte, M et al; A conduit system distributes chemokines and small blood-borne molecules through the splenic white pulp. J Exp Med 2003, 3: 505
5. Svensson, M et al; Stromal cells direct local differentiation of regulatory dendritic cells. Immunity 2004, 21: 505
6. Alba, R et al; Biodistribution and retargeting of FX-binding ablated adenovirus serotype 5 vectors. Blood 2010 (Epub july 7).
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Product Notes
The Fibroblasts (Catalog #AAA584267) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Fibroblasts, Mouse, mAb ER-TR7 reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Fibroblasts can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), Immunofluorescence (IF), P. Application Notes: F: Sections were stained using an indirect immunoperoxidase method (Ref.1). Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:20. Researchers should empirically determine the suitability of the Fibroblasts for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibroblasts, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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