Mouse Fibrillin Monoclonal Antibody | anti-tlr0419 antibody
Mouse Anti-Fibrillin-FITC
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
Fibrillin; Monoclonal Antibody; Mouse Anti-Fibrillin-FITC; Mouse Anti-Fibrillin - FITC; Mouse Anti-Fibrillin; anti-tlr0419 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
11C1.3
Specificity
Human and bovine fibrillin (Mr 350 kDa); other species not tested
The fibrillins are extracellular matrix glycoproteins which occur as a major component of a subset of connective tissue microfibrils. These microfibrils have a beaded appearance and a cross-sectional diameter of 10-12 nm. In elastic tissues, these structures are thought to provide the scaffold onto which elastin is assembled to form elastic fibers, although their function in non-elastic tissues is unclear.^1 In immunohistochemical staining of paraformaldehyde-fixed human skin, mAb 11C1.3 labels the elastic framework and more specifically the oxytalantic network close to the epidermis. The antibody also exhibits discrete staining of normal fibroblasts in culture and of extracellular (elastin) fibrils in a variety of tissues. ^2,3 There is an aberrant expression of fibrillin in Marfan syndrome. ^4
The fibrillins are extracellular matrix glycoproteins which occur as a major component of a subset of connective tissue microfibrils. These microfibrils have a beaded appearance and a cross-sectional diameter of 10-12 nm. In elastic tissues, these structures are thought to provide the scaffold onto which elastin is assembled to form elastic fibers, although their function in non-elastic tissues is unclear.^1 In immunohistochemical staining of paraformaldehyde-fixed human skin, mAb 11C1.3 labels the elastic framework and more specifically the oxytalantic network close to the epidermis. The antibody also exhibits discrete staining of normal fibroblasts in culture and of extracellular (elastin) fibrils in a variety of tissues. ^2,3 There is an aberrant expression of fibrillin in Marfan syndrome. ^4
Form/Format
Fluorescein (FITC) Conjugate
Sequence Length
193
Applicable Applications for anti-tlr0419 antibody
Immunofluorescence; Immunohistochemistry; Western Blot
Application Notes
Immunohistochemistry Suggested Dilution: 1:50-1:100 (30-60 min at RT)
Immunogen
Microfibrils from zonular apparatus of bovine eye
Characterization
To insure lot-to-lot consistency, each batch of product is tested by immunohistochemistry to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.2 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.2 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.2 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.2 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.2 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
References
1. Sakai, L.Y., et al. 1986. J. Cell. Biol. 103:2499.
2. Maier, A., et al. 1994. Investigative Opthamology and Visual Science 35:3103.
3. Wright, D.W., et al. 1994. Matrix Biol. 14:41.
4. Godfrey, M., et al. 1995. J. Amer. Acad. Dermatol. 56:1287.
2. Maier, A., et al. 1994. Investigative Opthamology and Visual Science 35:3103.
3. Wright, D.W., et al. 1994. Matrix Biol. 14:41.
4. Godfrey, M., et al. 1995. J. Amer. Acad. Dermatol. 56:1287.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
fibrillin
NCBI Official Symbol
tlr0419
NCBI Protein Information
fibrillin
Protein Family
Similar Products
Product Notes
The tlr0419 (Catalog #AAA673085) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Fibrillin can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence; Immunohistochemistry; Western Blot. Immunohistochemistry Suggested Dilution: 1:50-1:100 (30-60 min at RT). Researchers should empirically determine the suitability of the tlr0419 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibrillin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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